Please use this identifier to cite or link to this item: https://repositorio.ufrn.br/jspui/handle/123456789/25413
Title: Growth inhibitory effects of Phyllanthus niruri extracts in combination with cisplatin on cancer cell lines
Authors: Araújo, Aurigena Antunes de
Araújo Júnior, Raimundo Fernandes de
Soares, Luiz Alberto Lira
Porto, Cínthia Raquel da Costa
Aquino, Ranniere Gurgel Furtado de
Guedes, Hugo Gonçalo
Petrovick, Pedro Ros
Souza, Tatiane Pereira de
Guerra, Gerlane Coelho Bernardo
Keywords: Cisplatin;Colorectal cancer;Liver cancer;Phyllanthus niruri;Cytotoxic effect
Issue Date: 21-Aug-2012
Publisher: Baishideng
Citation: ARAÚJO, Aurigena Antunes de et al. Growth inhibitory effects of Phyllanthus niruri extracts in combination with cisplatin on cancer cell lines. World Journal of Gastroenterology, v. 18, p. 4162-4168, 2012. Disponível em: <https://www.wjgnet.com/1007-9327/full/v18/i31/4162.htm>. Acesso em: 14 mar. 2018.
Portuguese Abstract: AIM: To investigate the cytotoxic effects of spray-dried extracts of Phyllanthus niruri in combination with cisplatin on two cancer cell lines. METHODS: Colorectal carcinoma (HT29) and human hepatocellular carcinoma (HepG2) cells were treated with spray-dried extracts of Phyllanthus niruri (SDEPN) either alone or in combination with cisplatin at different concentrations (0.5 mg/mL and 1 mg/mL) for 4 h and 24 h. To verify and quantify cancer cells treated with these products as well as identify the cell cycle stage and cell viability, we stained the cells with propidium iodide and assessed them by flow cytometry. The percentage of cells in different cell cycle phases was quantified and data were expressed as histograms. Significant differences between groups were determined using analysis of variance and Bonferroni’s test, as indicated. A value of P < 0.05 was considered to be statistically significant. RESULTS: SDEPN had significantly different cytotoxic effects on HT29 (2.81 ± 0.11 vs 3.51 ± 1.13, P > 0.05) and HepG2 (5.07 ± 0.3 vs 15.9 ± 1.04, P < 0.001) cells when compared to control cells for 4 h. SDEPN also had significantly different cytotoxic effects on HT29 (1.91 ± 0.57 vs 4.53 ± 1.22, P > 0.05) and HepG2 (14.56 ± 1.6 vs 35.67 ± 3.94, P < 0.001) cells when compared to control cells for 24 h. Both cell lines were killed by cisplatin in a dose-dependent manner compared to control cells (HepG2 cells for 4 h: 10.78 ± 1.58 vs 53.89 ± 1.53, P < 0.001; 24 h: 8.9 ± 1.43 vs 62.78 ± 1.87, P < 0.001 and HT29 cells for 4 h: 9.52 ± 0.913 vs 49.86 ± 2.89, P < 0.001; 24 h: 11.78 ± 1.05 vs 53.34 ± 2.65, P < 0.001). In HT29 cells, pretreatment with SDEPN and subsequent treatment with cisplatin resulted in a greater number of cells being killed (12.78 ± 1.01 vs 93.76 ± 1.6, P < 0.001). HepG2 cells showed significant cell killing with treatment with SDEPN when combined with cisplatin (12.87 ± 2.78 vs 78.8 ± 3.02, P < 0.001). CONCLUSION: SDEPN is selectively toxic against two cancer cell lines. Moreover, SDEPN in combination with cisplatin induces a synergistic increase in the cell death of both HT29 and HepG2 cells.
URI: https://repositorio.ufrn.br/jspui/handle/123456789/25413
ISSN: 2219-2840
Appears in Collections:CB - DBF - Artigos publicados em periódicos

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