Navegando por Autor "Camillo, Christina S."
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Artigo Beneficial effects of tamarind trypsin inhibitor in chitosan–whey protein nanoparticles on hepatic injury induced high glycemic index diet: a preclinical study(International Journal Molecular Sciences, 2021) Morais, Ana Heloneida de Araujo; Aguiar, Ana J. F. C.; Queiroz, Jaluza L. C. de; Santos, Pedro P. A.; Camillo, Christina S.; Serquiz, Alexandre C.; Costa, Izael S.; Oliveira, Gerciane S.; Gomes, Ana F. T.; Matias, Lídia L. R.; Costa, Rafael O. A.; Passos, Thaís S.; https://orcid.org/0000-0002-6460-911XSeveral studies have sought new therapies for obesity and liver diseases. This study investigated the effect of the trypsin inhibitor isolated from tamarind seeds (TTI), nanoencapsulated in chitosan and whey protein isolate (ECW), on the liver health status of the Wistar rats fed with a high glycemic index (HGLI) diet. The nanoformulations without TTI (CW) and ECW were obtained by nanoprecipitation technique, physically and chemically characterized, and then administered to the animals. The adult male Wistar rats (n = 20) were allocated to four groups: HGLI diet + water; standard diet + water; HGLI diet + ECW (12.5 mg/kg); and HGLI diet + CW (10.0 mg/kg), 1 mL per gagave, for ten days. They were evaluated using biochemical and hematological parameters, Fibrosis 4 Index for Liver Fibrosis (FIB-4), AST to Platelet Ratio Index (APRI) scores, and liver morphology. Both nanoparticles presented spherical shape, smooth surface, and nanometric size [120.7 nm (ECW) and 136.4 nm (CW)]. In animals, ECW reduced (p < 0.05) blood glucose (17%), glutamic oxalacetic transaminase (39%), and alkaline phosphatase (24%). Besides, ECW reduced (p < 0.05) APRI and FIB-4 scores and presented a better aspect of hepatic morphology. ECW promoted benefits over a liver injury caused by the HGLI dietArtigo Tamarind multifunctional protein: safety and anti-inflammatory potential in intestinal mucosa and adipose tissue in a preclinical model of diet-induced obesity(Obesity Facts, 2021) Morais, Ana Heloneida de Araújo; Lima, Vanessa C. O.; Amarante, Maria do Socorro M.; Lima, Maíra C. J. S.; Carvalho, Fabiana M. C.; Figueredo, Julia B. S.; Santos, Pedro P. A.; Camillo, Christina S.; Ladd, Fernando V. L.; Maciel, Bruna L. L.; Uchôa, Adriana F.Introduction: Obesity has emerged as one of the main pub lic health problems. This condition triggers a series of hor monal and metabolic changes related to a low-grade chron ic inflammatory condition. The trypsin inhibitor purified from tamarind (TTIp) seeds is a promising anti-inflammatory molecule, but its safety needs to be evaluated. This study aimed to evaluate TTIp bioactive dose effects on organs in volved in its metabolism (liver and pancreas) and affected tissues (small intestine and perirenal adipose tissue) in an obesity model. Methods: Three groups of adult male Wistar rats were used (n = 5). Two of these groups had diet-induced obesity, and a third group was eutrophic. TTIp was adminis tered by gavage in one of the obese groups for 10 days, while the remaining groups received a vehicle. The chromato graphic profile and the inhibition assay corroded the purifi cation of the inhibitor. Physical and behavioral changes, liver enzymes, and stereological and histopathological analyses of tissues were evaluated. Results: TTIp did not cause visible signs of toxicity, nor caused changes in liver enzymes, the liver, and pancreatic tissues. TTIp did not cause changes in the intestinal mucosa, showing improvement in the villi’s histopathological characteristics compared to the group of animals with obesity without treatment with TTIp (p = 0.004). The analysis of perirenal adipose tissue showed that the av erage sectional area of animals with obesity that received TTIp did not differ from the control. There was a difference between the high glycemic load diet group and the group treated with the inhibitor (351.8 ± 55.5) (p = 0.016). In addi tion, the group that received TTIp had no inflammatory infil trates. Conclusion: Based on histological and stereological analysis, the use of TTIp is potentially safe and anti-inflam matory in the evaluated obesity model and can be investi gated as a possible adjuvant in obesity therapy.