Navegando por Autor "Carvalho, J. E."
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Artigo Anticancer activity of Tabernaemontana catharinensis extract obtained by supercritical fluid extraction(Sociedade Brasileira de Plantas Medicinais, 2006-10-31) Courtial, Camila Gambini Pereira; Carvalho, J. E.; Meireles, Maria Angela de AlmeidaIn the present work the anticancer of crude supercritical fluid extract from T. catharinensis was evaluated. The crude extract was obtained using supercritical CO² at pressure of 300 bar and temperature of 55º C; ethanol was used as cosolvent (4.6% mass). The anticancer activity was tested against seven human cancer cell lines: lung, breast, breast expressing rhe multidrug resistance phenotype, melanoma, colon, prostate, and, kidney. The crude extract was fractionated and the alkaloidal fraction was analyzed by thin-layer chromatografy (TLC), and gas-chromatography/flame ionization detector (GC-FID). The GC-FID of the alkaloidal fraction indicated the presence of the indole alkaloids voacangine and coronaridine. The crude extract of T. catharinensis has anticancer activity that begins at 0.25 ug/mL and exhibits cytostatic and cytolytic effects concentration dependent and noy selective for the cell lines used