Navegando por Autor "Maciel, Bruna L. L."
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Artigo Anti-Inflammatory protein isolated from tamarind promotes better histological aspects in the intestine regardless of the improvement of intestinal permeability in a preclinical study of diet-induced obesity(Nutrients, 2022) Morais, Ana Heloneida de Araújo; Lima, Mayara S. R.; Gonçalves, Catarina; D. Neto, Mafalda; Macedo, Maria Helena; Queiroz, Jaluza L. C. de; Silva, Valéria C. da; Costa, Izael de S.; Camillo, Christina da S.; Santos, Pedro Paulo de A.; Lima, Aldo A. M.; Pastrana, Lorenzo; Maciel, Bruna L. L.; https://orcid.org/0000-0002-6460-911XObesity is associated with metabolic and physiological effects in the gut. In this study, we evaluated the anti-inflammatory effect of trypsin inhibitor isolated from tamarind seeds (TTI) in vitro (interaction with lipopolysaccharide (LPS) and inhibitory activity against human neutrophil elastase (HNE)), and using intestinal co-cultures of Caco-2:HT29-MTX cell lines inflamed with TNF α (50 ng/mL) and a Wistar rat model of diet-induced obesity (n = 15). TTI was administered to animals by gavage (10 days), and the treated group (25 mg/kg/day) was compared to animals without treatment or treated with a nutritionally adequate diet. In the in vitro study, it showed inhibitory activity against HNE (93%). In co-cultures, there was no protection or recovery of the integrity of inflamed cell monolayers treated with TTI (1.0 mg/mL). In animals, TTI led to lower plasma concentrations of TNF-α and IL-6, total leukocytes, fasting glucose, and LDL-c (p < 0.05). The intestines demonstrated a lower degree of chronic enteritis, greater preservation of the submucosa, and greater intestinal wall thickness than the other groups (p = 0.042). Therefore, the better appearance of the intestine not reflected in the intestinal permeability added to the in vitro activity against HNE point to possibilities for new studies and applications related to this activity.Artigo Can probiotics and diet promote beneficial immune modulation and purine control in coronavirus infection?(Nutrients, 2020) Morais, Ana Heloneida de Araújo; Passos, Thais S.; Maciel, Bruna L. L.; Maia, Juliana K. da Silva; https://orcid.org/0000-0002-6460-911XInfection caused by the SARS-CoV-2 coronavirus worldwide has led the World Health Organization to declare a COVID-19 pandemic. Because there is no cure or treatment for this virus, it is emergingly urgent to find effective and validated methods to prevent and treat COVID-19 infection. In this context, alternatives related to nutritional therapy might help to control the infection. This narrative review proposes the importance and role of probiotics and diet as adjunct alternatives among the therapies available for the treatment of this new coronavirus. This review discusses the relationship between intestinal purine metabolism and the use of Lactobacillus gasseri and low-purine diets, particularly in individuals with hyperuricemia, as adjuvant nutritional therapies to improve the immune system and weaken viral replication, assisting in the treatment of COVID-19. These might be promising alternatives, in addition to many others that involve adequate intake of vitamins, minerals and bioactive compounds from foodArtigo Tamarind (Tamarindus indica L.) seed a candidate protein source with potential for combating SARS-CoV-2 infection in obesity(Drug Target Insights, 2021) Morais, Ana Heloneida de Araújo; Medeiros, Amanda F. de; Medeiros, Isaiane; Lima, Vanessa C. O. de; Luz, Anna B. S.; Maciel, Bruna L. L.; Passos, Thaís S.; https://orcid.org/0000-0002-6460-911XIntroduction: Obesity and coronavirus disease (COVID)-19 are overlapping pandemics, and one might worsen the other. Methods: This narrative review discusses one of the primary mechanisms to initiate acute respiratory distress syndrome, uncontrolled systemic inflammation in COVID-19, and presents a potential candidate for adjuvant treatment. Blocking the S protein binding to angiotensin-converting enzyme 2 (ACE-2) and the 3C-like protease (3CL pro) is an effective strategy against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Results: Host proteases such as FURIN, trypsin, and transmembrane serine protease 2 (TMPRSS) act in S protein activation. Tamarind trypsin inhibitor (TTI) shows several beneficial effects on the reduction of inflammatory markers (tumor necrosis factor α [TNF-α], leptin) and biochemical parameters (fasting glycemia, triglycerides, and very low-density lipoprotein [VLDL]), in addition to improving pancreatic function and mucosal integrity in an obesity model. TTI may inhibit the action of proteases that collaborate with SARS-CoV-2 infection and the neutro phil activity characteristic of lung injury promoted by the virus. Conclusion: Thus, TTI may contribute to combating two severe overlapping problems with high cost and social complex implications, obesity and COVID-19.Artigo Tamarind multifunctional protein: safety and anti-inflammatory potential in intestinal mucosa and adipose tissue in a preclinical model of diet-induced obesity(Obesity Facts, 2021) Morais, Ana Heloneida de Araújo; Lima, Vanessa C. O.; Amarante, Maria do Socorro M.; Lima, Maíra C. J. S.; Carvalho, Fabiana M. C.; Figueredo, Julia B. S.; Santos, Pedro P. A.; Camillo, Christina S.; Ladd, Fernando V. L.; Maciel, Bruna L. L.; Uchôa, Adriana F.Introduction: Obesity has emerged as one of the main pub lic health problems. This condition triggers a series of hor monal and metabolic changes related to a low-grade chron ic inflammatory condition. The trypsin inhibitor purified from tamarind (TTIp) seeds is a promising anti-inflammatory molecule, but its safety needs to be evaluated. This study aimed to evaluate TTIp bioactive dose effects on organs in volved in its metabolism (liver and pancreas) and affected tissues (small intestine and perirenal adipose tissue) in an obesity model. Methods: Three groups of adult male Wistar rats were used (n = 5). Two of these groups had diet-induced obesity, and a third group was eutrophic. TTIp was adminis tered by gavage in one of the obese groups for 10 days, while the remaining groups received a vehicle. The chromato graphic profile and the inhibition assay corroded the purifi cation of the inhibitor. Physical and behavioral changes, liver enzymes, and stereological and histopathological analyses of tissues were evaluated. Results: TTIp did not cause visible signs of toxicity, nor caused changes in liver enzymes, the liver, and pancreatic tissues. TTIp did not cause changes in the intestinal mucosa, showing improvement in the villi’s histopathological characteristics compared to the group of animals with obesity without treatment with TTIp (p = 0.004). The analysis of perirenal adipose tissue showed that the av erage sectional area of animals with obesity that received TTIp did not differ from the control. There was a difference between the high glycemic load diet group and the group treated with the inhibitor (351.8 ± 55.5) (p = 0.016). In addi tion, the group that received TTIp had no inflammatory infil trates. Conclusion: Based on histological and stereological analysis, the use of TTIp is potentially safe and anti-inflam matory in the evaluated obesity model and can be investi gated as a possible adjuvant in obesity therapy.Artigo Validity of bioelectrical impedance to estimate fat-free mass in boys with Duchenne muscular dystrophy(Plos One, 2020) Dourado Junior, Mário Emílio Teixeira; Grilo, Evellyn C.; Cunha, Thais A.; Costa, Ádila Danielly S.; Araújo, Bárbara G. M.; Lopes, Márcia Marília G. D.; Maciel, Bruna L. L.; Alves, Camila X.; Serpa, Karina M. Vermeulen; Lais, Lucia Leite; Brandão-Neto, José; Vale, Sancha Helena L.; https://orcid.org/0000-0002-9462-2294The evaluation of fat-free mass (FFM) in patients with Duchenne muscular dystrophy (DMD) is useful to investigate disease progression and therapeutic efficacy. This study aimed to validate the Bioelectrical impedance (BIA) method compared with the dual-energy X-ray absorptiometry (DXA) for estimating the %FFM in boys with DMD. This is a cross-sectional study performed with children and adolescents diagnosed with DMD. Resistance and reactance were measured with a BIA analyzer, from which eight predictive equations estimated the %FFM. The %FFM was also determined by DXA and its used as a reference method. Pearson correlation test, coefficient of determination, the root-mean-square error, the interclass correlation coefficient, and linear regression analysis were performed between %FFM values obtained by BIA and DXA. The agreement between these values was verified with the Bland-Altman plot analysis. Forty-six boys aged from 5 to 20 years were enrolled in the study. All the equations showed a correlation between the %FFM estimated by BIA and determined by DXA (p < 0.05). The Bland-Altman method indicated that two equations have a significant bias (p < 0.05) and six equations showed no significant bias of %FFM (p > 0.05). However, one of them has high variation and wide limits of agreement. Five of eight %FFM predictive equations tested in DMD were accurate when compared with the DXA. It can be concluded that BIA is a validity method to evaluate patients with DMD.