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Title: Biodistribution of the radiophamarceutical sodium pertechnetate (Na99mTcO4) after massive small bowel resection in rats
Other Titles: Biodistribuição do radiofármaco pertecnetato de sódio (Na99mTcO4) em ratos submetidos a ressecção extensa de intestino delgado
Authors: Chacon, Dâmaso de Araújo
Araújo Filho, Irami
Villarim Neto, Arthur
Rêgo, Amália Cínthia Meneses
Azevedo, Ítalo Medeiros
Bernardo Filho, Mário
Brandão Neto, José
Medeiros, Aldo Cunha
Keywords: Short Bowel Syndrome;Sodium Pertechnetate Tc 99m;Pharmacokinetics;Rat
Issue Date: 2007
Citation: CHACON, Dâmaso de Araújo; ARAÚJO FILHO, Irami; VILLARIM NETO, Arthur; RÊGO, Amália Cínthia Meneses; AZEVEDO, Ítalo Medeiros; BERNARDO FILHO, Mário; BRANDÃO NETO, José; MEDEIROS, Aldo Cunha. Biodistribution of the radiophamarceutical sodium pertechnetate (Na99mTcO4) after massive small bowel resection in rats. Acta Cirúrgica Brasileira , v. 22, p. 430-435, 2007. Disponível em: <> Acesso em: 09 out. 2013.
Abstract: To evaluate the biodistribution of sodium pertecnetate (Na99mTcO4) in organs and tissues, the morphometry of remnant intestinal mucosa and ponderal evolution in rats subjected to massive resection of the small intestine. Methods: Twenty-one Wistar rats were randomly divided into three groups of 7 animals each. The short bowel (SB) group was subjected to massive resection of the small intestine; the control group (C) rats were not operated on, and soft intestinal handling was performed in sham rats. The animals were weighed weekly. On the 30th postoperative day, 0.l mL of Na99mTcO4, with mean activity of 0.66 MBq was injected intravenously into the orbital plexus. After 30 minutes, the rats were killed with an overdose of anesthetic, and fragments of the liver, spleen, pancreas, stomach, duodenum, small intestine, thyroid, lung, heart, kidney, bladder, muscle, femur and brain were harvested. The biopsies were washed with 0.9% NaCl.,The radioactivity was counted using Gama Counter WizardTM 1470, PerkinElmer. The percentage of radioactivity per gram of tissue (%ATI-g) was calculated. Biopsies of the remaining jejunum were analysed by HE staining to obtain mucosal thickness. Analysis of variance (ANOVA) and the Tukey test for multiple comparisons were used, considering p<0.05 as signifi cant. Results: There were no signifi cant differences in %ATI-g of the Na99mTcO4 in the organs of the groups studied (p>0.05). An increase in the weight of the SB rats was observed after the second postoperative week. The jejunal mucosal thickness of the SB rats was signifi cantly greater than that of C and sham rats (p<0.05). Conclusion: In rats with experimentally-produced short bowel syndrome, an adaptive response by the intestinal mucosa reduced weight loss. The biodistribution of Na99mTcO4 was not affected by massive intestinal resection, suggesting that short bowel syndrome is not the cause of misleading interpretation, if an examination using this radiopharmaceutical is indicated
ISSN: 1678-2674
Appears in Collections:CCS - DCIRUR - Artigos publicados em periódicos

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