Antioxidant fucoidans obtained from tropical seaweed protect pre-osteoblastic cells from hydrogen peroxide-induced damage

Fidelis, Gabriel Pereira; Silva, Cynthia Haynara Ferreira; Nobre, Leonardo Thiago Duarte Barreto; Medeiros, Valquíria Pereira; Rocha, Hugo Alexandre de Oliveira; Costa, Leandro Silva

Antioxidant fucoidans obtained from tropical seaweed protect pre-osteoblastic cells from hydrogen peroxide-induced damage

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dc.contributor.author	Fidelis, Gabriel Pereira
dc.contributor.author	Silva, Cynthia Haynara Ferreira
dc.contributor.author	Nobre, Leonardo Thiago Duarte Barreto
dc.contributor.author	Medeiros, Valquíria Pereira
dc.contributor.author	Rocha, Hugo Alexandre de Oliveira
dc.contributor.author	Costa, Leandro Silva
dc.date.accessioned	2020-12-29T14:28:13Z
dc.date.available	2020-12-29T14:28:13Z
dc.date.issued	2019-08-28
dc.description.resumo	Some antioxidant compounds decrease the amount of intracellular reactive oxygen species (ROS) and consequently reduce the deleterious effects of ROS in osteoblasts. Thus, these compounds fight against osteoporosis. Brown seaweeds are a rich source of antioxidant fucose-containing sulfated polysaccharides (fucans and fucoidans). We obtained six fucoidans (FRFs)—F0.3, F0.5, F0.7, F1.0, F1.5, and F2.1—from Dictyota mertensii by proteolytic digestion followed by sequential acetone precipitation. Except for F0.3, all FRFs showed antioxidant activity in different in vitro tests. In pre- osteoblast-like cells (MC3T3-L1) exposed to H2O2-oxidative stress, caspase-3 and caspase-9 were activated, resulting in apoptosis of the cells. We also observed a decrease in superoxide dismutase (SOD) and alkaline phosphatase (ALP) activity. The antioxidant FRFs protected the cells from the oxidative damage caused by H2O2, decreasing intracellular ROS and caspase activation, and increasing SOD activity. The most effective protection against damage was provided by F0.7, F1.5, and F2.1. At 0.5 mg/mL, these FRFs also suppressed the H2O2-mediated inhibition of ALP activity. The data indicated that FRFs F0.7, F1.5, and F2.1 from D. mertensii were antioxidants that protected bone tissue from oxidative stress and could represent possible adjuvants for the treatment of bone fragility through counteracting oxidative phenomena	pt_BR
dc.identifier.citation	FIDELIS, Gabriel Pereira; SILVA, Cynthia Haynara Ferreira; NOBRE, Leonardo Thiago Duarte Barreto; MEDEIROS, Valquíria Pereira; ROCHA, Hugo Alexandre de Oliveira; COSTA, Leandro Silva. Antioxidant fucoidans obtained from tropical seaweed protect pre-osteoblastic cells from hydrogen peroxide-induced damage. Marine Drugs, [s. l.], v. 17, n. 9, p. 506-523, 28 ago. 2019. MDPI AG. Disponível em: https://www.mdpi.com/1660-3397/17/9/506. Acesso em: 16 set. 2020. http://dx.doi.org/10.3390/md17090506.	pt_BR
dc.identifier.doi	10.3390/md17090506
dc.identifier.issn	1660-3397 (online)
dc.identifier.uri	https://repositorio.ufrn.br/handle/123456789/31146
dc.language	en	pt_BR
dc.publisher	MDPI	pt_BR
dc.rights	Attribution 3.0 Brazil	*
dc.rights.uri	http://creativecommons.org/licenses/by/3.0/br/	*
dc.subject	Bone cells	pt_BR
dc.subject	Sulfated polysaccharides	pt_BR
dc.subject	Osteoporosis	pt_BR
dc.subject	Brown seaweed	pt_BR
dc.subject	Oxidative damage	pt_BR
dc.title	Antioxidant fucoidans obtained from tropical seaweed protect pre-osteoblastic cells from hydrogen peroxide-induced damage	pt_BR
dc.type	article	pt_BR