Continuous live imaging of adult neural stem cell division and lineage progression in vitro

Costa, Marcos Romualdo; Ortega, Felipe; Brill, Monika S.; Beckervordersandforth, Ruth; Petrone, Ciro; Schroeder, Timm; Götz, Magdalena; Berninger, Benedikt

Continuous live imaging of adult neural stem cell division and lineage progression in vitro

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dc.contributor.author	Costa, Marcos Romualdo
dc.contributor.author	Ortega, Felipe
dc.contributor.author	Brill, Monika S.
dc.contributor.author	Beckervordersandforth, Ruth
dc.contributor.author	Petrone, Ciro
dc.contributor.author	Schroeder, Timm
dc.contributor.author	Götz, Magdalena
dc.contributor.author	Berninger, Benedikt
dc.date.accessioned	2017-05-24T16:54:20Z
dc.date.available	2017-05-24T16:54:20Z
dc.date.issued	2011
dc.description.resumo	Little is known about the intrinsic specification of adult neural stem cells (NSCs) and to what extent they depend on their local niche. To observe adult NSC division and lineage progression independent of their niche, we isolated cells from the adult mouse subependymal zone (SEZ) and cultured them at low density without growth factors. We demonstrate here that SEZ cells in this culture system are primarily neurogenic and that adult NSCs progress through stereotypic lineage trees consisting of asymmetric stem cell divisions, symmetric transit-amplifying divisions and final symmetric neurogenic divisions. Stem cells, identified by their astro/radial glial identity and their slow-dividing nature, were observed to generate asymmetrically and fast-dividing cells that maintained an astro/radial glia identity. These, in turn, gave rise to symmetrically and fast-dividing cells that lost glial hallmarks, but had not yet acquired neuronal features. The number of amplifying divisions was limited to a maximum of five in this system. Moreover, we found that cell growth correlated with the number of subsequent divisions of SEZ cells, with slow-dividing astro/radial glia exhibiting the most substantial growth prior to division. The fact that in the absence both of exogenously supplied growth factors and of signals provided by the local niche neurogenic lineage progression takes place in such stereotypic fashion, suggests that lineage progression is, to a significant degree, cell intrinsic or pre-programmed at the beginning of the lineage.	pt_BR
dc.identifier.doi	10.1242/dev.061663
dc.identifier.issn	0950-1991
dc.identifier.uri	https://repositorio.ufrn.br/jspui/handle/123456789/23066
dc.language	eng	pt_BR
dc.rights	Acesso Aberto	pt_BR
dc.subject	Asymmetric cell division	pt_BR
dc.subject	Astroglia	pt_BR
dc.subject	Neurogenesis	pt_BR
dc.subject	Radial glia	pt_BR
dc.subject	Subependymal zone	pt_BR
dc.subject	Time-lapse video microscopy	pt_BR
dc.subject	Mouse	pt_BR
dc.title	Continuous live imaging of adult neural stem cell division and lineage progression in vitro	pt_BR
dc.type	article	pt_BR