Modeling and simulation of Bacillus cereus chitosanase activity during purification using expanded bed chromatography

Página do item simplificado Estatísticas
dc.contributor.authorSouza, Domingos Fabiano de Santana
dc.contributor.authorPadilha, Carlos Eduardo de Araújo
dc.contributor.authorAraújo, Nathália Kelly de
dc.contributor.authorOliveira, Jackson Araújo de
dc.contributor.authorMacedo, Gorete Ribeiro de
dc.contributor.authorSantos, Everaldo Silvino dos
dc.date.accessioned2021-12-06T18:24:29Z
dc.date.available2021-12-06T18:24:29Z
dc.date.issued2016-07
dc.description.resumo−A phenomenological model was used to describe sequentially the three steps (flowthrough, washing and elution) of expanded bed adsorption chromatography for recovery of chitosanases from Bacillus cereus. Additionally, a hybrid strategy for model parameter estimation was carried out using particle swarm optimization and Gauss-Newton algorithms. The model was validated with independent experimental data and the statistical criteria (χ2 and mean squared error tests) showed that the hybrid strategy was more promising than just the heuristic method. With the calibrated model, surface response methodology was applied to obtain the optimal operational conditions, and experiments were performed to confirm these results. Overall, a value of 41.08% for yield was obtained using 700 mM NaCl during elution. In summary, all approach employed in this work was relevant for maximizing the yield of the chromatographic processpt_BR
dc.identifier.citationPADILHA, Carlos Eduardo de Araújo; ARAðJO, Nathália Kelly de; SOUZA, Domingos Fabiano de Santana; OLIVEIRA, Jackson Araújo de; MACEDO, Gorete Ribeiro de; SANTOS, Everaldo Silvino dos. Modeling and simulation of Bacillus cereus chitosanase activity during purification using expanded bed chromatography. Korean Journal Of Chemical Engineering, [S.L.], v. 33, n. 9, p. 2650-2658, 18 jul. 2016. Springer Science and Business Media LLC. http://dx.doi.org/10.1007/s11814-016-0127-1. Disponível em: https://link.springer.com/article/10.1007/s11814-016-0127-1. Acesso em: 05 nov. 2021.pt_BR
dc.identifier.doi10.1007/s11814-016-0127-1.
dc.identifier.issn1975-7220
dc.identifier.urihttps://repositorio.ufrn.br/handle/123456789/45193
dc.languageenpt_BR
dc.publisherSpringerpt_BR
dc.subjectModelingpt_BR
dc.subjectExpanded bed adsorptionpt_BR
dc.subjectChitosanasespt_BR
dc.subjectParticle swarm optimizationpt_BR
dc.subjectGauss-Newtonpt_BR
dc.subjectOptimizationpt_BR
dc.titleModeling and simulation of Bacillus cereus chitosanase activity during purification using expanded bed chromatographypt_BR
dc.typearticlept_BR

Arquivos

Pacote Original

Agora exibindo 1 - 1 de 1
Nenhuma Miniatura disponível
Nome:
ModelingSimulationBacillus_Souza_2016.pdf
Tamanho:
659.11 KB
Formato:
Adobe Portable Document Format
Nenhuma Miniatura disponível
Baixar

Licença do Pacote

Agora exibindo 1 - 1 de 1
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
1.45 KB
Formato:
Item-specific license agreed upon to submission
Nenhuma Miniatura disponível
Baixar

Coleções

CT - DEQ - Artigos publicados em periódicos
Repositório Institucional - UFRN Campus Universitário Lagoa NovaCEP 59078-970 Caixa postal 1524 Natal/RN - BrasilUniversidade Federal do Rio Grande do Norte© Copyright 2025. Todos os direitos reservados.
Contato+55 (84) 3342-2260 - R232Setor de Repositórios Digitaisrepositorio@bczm.ufrn.br
DSpaceIBICT
OasisBR
LAReferencia
Customizado pela CAT - BCZM