Montagem e anotação de genomas mitocondriais amazônicos a partir de dados públicos disponíveis no NCBI

Abstract

Technological advances in recent years have enabled the development of technologies that facilitate the sequencing and storage of genomic data in public databases, such as the NCBI. However, species from the Global South, specifically Amazonian species, are poorly represented in public databases. Therefore, in order to reduce this gap, this study aims to assemble and annotate 100 mitochondrial genomes of Amazonian fish using public data available at NCBI, contributing data from 22 new species that were not present in GenBank. The accuracy of the assemblies was validated through sequence identity comparisons with reference mitogenomes, reaching up to 100% identity for the species Pygocentrus nattereri (Kner, 1858). In addition, possible mislabeling of species present in public databases was also found. By expanding the genomic data of Amazonian fish, this study helps to fill significant gaps in genomic databases, contributing to new studies on conservation, sustainable fisheries management and the biodiversity of Amazonian fish. In addition to making the results available in public databases, the data will also be deposited on a website dedicated to gathering genomic data from Amazonian species with the aim of democratizing access to genomic data.