Using an adherent cell culture of the mouse subependymal zone to study the behavior of adult neural stem cells on a single-cell level

Ortega, Felipe; Costa, Marcos Romualdo; Simon-Ebert, Tatiana; Schroeder, Timm; Götz, Magdalena

Using an adherent cell culture of the mouse subependymal zone to study the behavior of adult neural stem cells on a single-cell level

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dc.contributor.author	Ortega, Felipe
dc.contributor.author	Costa, Marcos Romualdo
dc.contributor.author	Simon-Ebert, Tatiana
dc.contributor.author	Schroeder, Timm
dc.contributor.author	Götz, Magdalena
dc.date.accessioned	2017-05-26T18:25:46Z
dc.date.available	2017-05-26T18:25:46Z
dc.date.issued	2011-11-03
dc.description.resumo	A comprehensive understanding of the cell biology of adult neural stem cells (aNSCNSCNSCs) requires direct observation of aNSCNSCNSC division and lineage progression in the absence of niche-dependent signals. Here we describe a culture preparation of the adult mouse subependymal zone (SESEZ), which allows for continuous single-cell tracking of aNSCNSCNSC behavior. The protocol involves the isolation (~3 h) and culture of cells from the adult SESEZ at low density in the absence of mitogenic growth factors in chemically defined medium and subsequent live imaging using time-lapse video microscopy (5–7 d); these steps are followed by postimaging immunocytochemistry to identify progeny (~7 h). This protocol enables the observation of the progression from slow-dividing aNSCNSCNSCs of radial/astroglial identity up to the neuroblast stage, involving asymmetric and symmetric cell divisions of distinct fast-dividing precursors. This culture provides an experimental system for studying instructive or permissive effects of signal molecules on aNSCNSCNSC modes of cell division and lineage progression.	pt_BR
dc.identifier.issn	1754-2189
dc.identifier.uri	https://repositorio.ufrn.br/jspui/handle/123456789/23135
dc.language	eng	pt_BR
dc.rights	Acesso Aberto	pt_BR
dc.subject	adult neural stem cells	pt_BR
dc.subject	single-cell level	pt_BR
dc.subject	cell culture	pt_BR
dc.subject	mouse	pt_BR
dc.title	Using an adherent cell culture of the mouse subependymal zone to study the behavior of adult neural stem cells on a single-cell level	pt_BR
dc.type	article	pt_BR