Optimization of separation of bioactive compounds in daily foods by aqueous two-phase flotation coupled with HPLC-DAD

Santos, Everaldo Silvino dos; Padilha, Carlos Eduardo de Araújo; Nogueira, Cleitiane da Costa; Leitão, Ana Laura de Sá; Rocha, Patrícia Maria; Almeida, Hanna Nóbrega; Souza, Domingos Fabiano Santana; Oliveira, Jackson Araújo de; Macedo, Gorete Ribeiro de

Optimization of separation of bioactive compounds in daily foods by aqueous two-phase flotation coupled with HPLC-DAD

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dc.contributor.author	Santos, Everaldo Silvino dos
dc.contributor.author	Padilha, Carlos Eduardo de Araújo
dc.contributor.author	Nogueira, Cleitiane da Costa
dc.contributor.author	Leitão, Ana Laura de Sá
dc.contributor.author	Rocha, Patrícia Maria
dc.contributor.author	Almeida, Hanna Nóbrega
dc.contributor.author	Souza, Domingos Fabiano Santana
dc.contributor.author	Oliveira, Jackson Araújo de
dc.contributor.author	Macedo, Gorete Ribeiro de
dc.date.accessioned	2021-04-26T14:43:01Z
dc.date.available	2021-04-26T14:43:01Z
dc.date.issued	2017-06-10
dc.description.resumo	In this study, we have used Aqueous Two-Phase Flotation (ATPF) in order to recovery and concentrate bioactive phenolic compounds found in foods. Firstly, the effects of the operational conditions on the ATPF performance have been investigated using a model solution of Gallic Acid (GA). The optimal conditions were: [ammonium sulfate] = 450 g/L, pH = 3.8, air flowrate = 37.5 mL/min, time flotation = 120 min, initial organic phase volume = 10.0 mL and polyethylene glycol 400 as solvent. Next, experiments were carried out with aqueous extracts of daily foods and the ATPF phases were analyzed by HPLC-DAD. In terms of total phenolic compounds content, the highest percentage of separation was obtained for clove extract with 38.80% due to transfer of gallic acid and eugenol to PEG rich-phase. The results showed that ATPF is a promising technique for be applied for separation and purification of phenolic compounds mainly in natural extracts	pt_BR
dc.identifier.citation	PADILHA, C. E.; NOGUEIRA, C. C.; LEITAO, A. L. S.; ROCHA, P. M.; ALMEIDA, H. N.; SOUZA, D. S. S.; OLIVEIRA, J. A.; MACEDO, G. R.; SANTOS, E. S.. Optimization of Separation of Bioactive Compounds in Daily Foods by Aqueous Two-Phase Flotation Coupled With HPLC-DADOptimization of Separation of Bioactive Compounds in Daily Foods by Aqueous Two-Phase Flotation Coupled With HPLC-DAD. International Journal of Chromatography and Separation Techniques, v. 106, p. 1-8, 2017. Disponível em https://gavinpublishers.com/articles/research-article/International-Journal-of-Chromatography-and-Separation-Techniques/optimization-of-separation-of-bioactive-compounds-in-daily-foods-by-aqueous-two-phase-flotation-coupled-with-hplc-dad. Acesso em: 25 mar. 2021. http://www.doi.org/10.29011/IJCST-106.000006.	pt_BR
dc.identifier.doi	10.29011/IJCST-106.000006
dc.identifier.issn	2577-218X
dc.identifier.uri	https://repositorio.ufrn.br/handle/123456789/32317
dc.language	en	pt_BR
dc.publisher	Gavin Publishers	pt_BR
dc.rights	Attribution-ShareAlike 3.0 Brazil	*
dc.rights.uri	http://creativecommons.org/licenses/by-sa/3.0/br/	*
dc.subject	Aqueous Two-Phase Flotation	pt_BR
dc.subject	Concentration	pt_BR
dc.subject	Foods	pt_BR
dc.subject	Gallic Acid	pt_BR
dc.subject	Polyethylene Glycol	pt_BR
dc.subject	Recovery	pt_BR
dc.title	Optimization of separation of bioactive compounds in daily foods by aqueous two-phase flotation coupled with HPLC-DAD	pt_BR
dc.type	article	pt_BR