Efeito da membrana quitosana com complexo de Zinco-vanilina no tratamento de lesões cutâneas em ratos diabéticos

Abstract

The treatment of chronic wounds is still considered a public health problem, when it affects groups of risks as diabetics and becomes a great clinical challenge. In this work, we evaluated the healing effects of a new zinc complex associated with a chitosan membrane, tested in cutaneous wounds of diabetic rats. Wistar rats were submitted to induction of diabetes with streptozotocin 40 mg / kg (i.v.). On the seventh day after diabetic induction, a circular skin excision (1.0 cm) with punch was performed. The lesions were treated with the pure chitosan membrane and with the membrane associated with the Zinc-vanillin complex (CS-ZPV1 and CS-ZPV2) at two concentrations, 7.36 x 10-7 mol cm-2 and 1.22 x 10-6 mol cm -2, respectively. The samples were submitted to macroscopic, histopathological, cytokine (TNFα, IL-1β and IL-10) and reverse transcriptase polymerase chain reaction (RtPCR) (TGFβ and VEGF) assays. The analysis revealed reepithelialization, angiogenic stimulation, collagen deposition and expression of proteins that aid in the healing process in a significant proportion in the groups treated with the CS-ZPV membrane compared to the groups without treatment. Treatment with CS-ZPV reduced the levels of TNF-α, IL-1β and increased IL-10, also increased the gene expression of TGFβ and VEGF. The evaluated parameters suggest that CSZPV in the two concentrations tested may be effective in the treatment of chronic wounds.