1. Universidade Federal do Rio Grande do Norte
  2. Instituto do Cérebro
  3. ICe - Artigos publicados em periódicos
Use este identificador para citar ou linkar para este item: https://repositorio.ufrn.br/handle/123456789/26786
Registro completo de metadados
Campo DCValorIdioma
dc.contributor.authorOliveira, Ramatis B. de-
dc.contributor.authorPetiz, Lyvia L.-
dc.contributor.authorLim, Rebecca-
dc.contributor.authorLipski, Janusz-
dc.contributor.authorGravina, Fernanda S.-
dc.contributor.authorBrichta, Alan M.-
dc.contributor.authorCallister, Robert J.-
dc.contributor.authorLeão, Richardson Naves-
dc.contributor.authorvan Helden, Dirk F.-
dc.date.accessioned2019-03-15T12:24:53Z-
dc.date.available2019-03-15T12:24:53Z-
dc.date.issued2019-03-03-
dc.identifier.citationOLIVEIRA, R. B. et al. Crosstalk between mitochondria, calcium channels and actin cytoskeleton modulates noradrenergic activity of locus coeruleus neurons. J. Neurochem., mar. 2019. doi: 10.1111/jnc.14692pt_BR
dc.identifier.urihttps://repositorio.ufrn.br/jspui/handle/123456789/26786-
dc.languageenpt_BR
dc.subjectLocus coeruleuspt_BR
dc.subjectnoradrenergic neuronspt_BR
dc.titleCrosstalk between mitochondria, calcium channels and actin cytoskeleton modulates noradrenergic activity of locus coeruleus neuronspt_BR
dc.typearticlept_BR
dc.identifier.doi10.1111/jnc.14692-
dc.description.resumoLocus coeruleus (LC) is the name of a group of large sized neurons located at the brain stem, which provide the main source of noradrenaline to the central nervous system, virtually, innervating the whole brain. All noradrenergic signalling provided by this nucleus is dependent on an intrinsic pacemaker process. Our study aims to understand how noradrenergic neurons finely tune their pacemaker processes and regulate their activities. Here we present that mitochondrial perturbation in the LC from mice, inhibits spontaneous firing by a hyperpolarizing response that involves Ca2+ entry via L‐type Ca2+ channels and the actin cytoskeleton. We found that pharmacological perturbation of mitochondria from LC neurons using the protonophore carbonyl cyanide m‐chlorophenylhydrazone (CCCP), induced a dominant hyperpolarizing response when electrophysiological approaches were performed. Surprisingly, the CCCP‐induced hyperpolarizing response was dependent on L‐type Ca2+ channel‐mediated Ca2+ entry, as it was inhibited by: removal of extracellular Ca2+; addition of Cd2+; nifedipine or nicardipine; but not by intracellular dialysis with the Ca2+ chelator BAPTA, the latter indicating that the response was not due to a global change in [Ca2+]c but does not exclude action at intracellular microdomains. Further to this, incubation of slices with cytochalasin D, an agent that depolymerises the actin cytoskeleton, inhibited the hyperpolarizing response indicating an involvement of the actin cytoskeleton. The data are consistent with the hypothesis that there is crosstalk between mitochondria and L‐type Ca2+ channels leading to modulation of noradrenergic neuronal activity mediated by the actin cytoskeleton.pt_BR
Aparece nas coleções:ICe - Artigos publicados em periódicos

Arquivos associados a este item:
Não existem arquivos associados a este item.
Mostrar registro simples do item


Os itens no repositório estão protegidos por copyright, com todos os direitos reservados, salvo quando é indicado o contrário.