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https://repositorio.ufrn.br/handle/123456789/32543| Título: | Production, recovery, and purification of recombinant 503 antigen of Leishmania infantum chagasi using expanded bed adsorption chromatography |
| Autor(es): | Santos, Everaldo Silvino dos Sousa Junior, Francisco Canindé de Vaz, Michelle Rossana Ferreira Padilha, Carlos Eduardo de Araújo Martins, Daniella Regina Arantes Macedo, Gorete Ribeiro de |
| Palavras-chave: | Visceral Leishmaniasis;Leishmaniasis;Elution Condition;Batch Adsorption Experiment;Leishmania Infantum |
| Data do documento: | 1-Out-2014 |
| Editor: | BMC |
| Referência: | SOUSA JUNIOR, Francisco Canindé de; VAZ, Michelle Rossana Ferreira; PADILHA, Carlos Eduardo de Araújo; MARTINS, Daniella Regina Arantes; MACEDO, Gorete Ribeiro de; SANTOS, Everaldo Silvino dos. Production, recovery, and purification of recombinant 503 antigen of Leishmania infantum chagasi using expanded bed adsorption chromatography. Bmc Proceedings, [S.L.], v. 8, n. 4, out. 2014. Disponível em: https://bmcproc.biomedcentral.com/articles/10.1186/1753-6561-8-S4-P194. Acesso em: 08 abr. 2021. https://doi.org/10.1186/1753-6561-8-S4-P194 |
| Resumo: | Visceral leishmaniasis, a disease caused by Leishmania infantum chagasi, represents a major public health problem in many areas of the world. Despite the considerable effort, there is no effective and safe vaccine for human use [1]. Some authors have reported that as much as 50% of overall costs in the biotechnology industries are related to downstream processing. Thus, the development of new and economically advantageous purification methods is a challenge [2]. Expanded bed adsorption (EBA) is an innovative chromatography technology that allows the adsorption of target proteins directly from unclarified feedstock. EBA technology combines solid-liquid separation with an adsorption step in a single-unit operation, aiming at increased overall yield, reduced operational time, and less capital investment and consumables [3,4]. Thus, the aim of this work was to purify the 503 antigen of Leishmania i. chagasi directly from crude feedstock using EBA chromatography |
| URI: | https://repositorio.ufrn.br/handle/123456789/32543 |
| ISSN: | 1753-6561 |
| Aparece nas coleções: | CCS - DFAR - Artigos publicados em periódicos CT - DEQ - Artigos publicados em periódicos |
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| ProductionRecoveryPurification_Santos_2014.pdf | 251,72 kB | Adobe PDF | Visualizar/Abrir |
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