Ferrari, MárcioPinheiro, Jonas Gabriel de Oliveira2025-05-302025-05-302024-12-19PINHEIRO, Jonas Gabriel de Oliveira. Desenvolvimento de bioingrediente multifuncional para indústria cosmética a partir do subproduto industrial de Agave sisalana (SISAL). Orientador: Dr. Márcio Ferrari. 2024. 104f. Tese (Doutorado em Desenvolvimento e Inovação Tecnológica em Medicamentos) - Centro de Ciências da Saúde, Universidade Federal do Rio Grande do Norte, Natal, 2024.https://repositorio.ufrn.br/handle/123456789/63770Agave sisalana Perrine (sisal) is a plant extensively cultured in Caatinga, most prevalent biome in northeastern Brazil. Fiber obtaining is the main objective. Only 5 % to 6 % of leaf’s crude weight are used in the defibration process resulting in a considerable amount byproduct, which is often dropped in the ground, which may represent an opportunity to take advantage of this by-product. The chemical composition and different activities already were studied in extracts from sisal byproduct, which motivated the development of new extracts and search for new activities. The aim of this study was to develop a multifunctional bioingredient with applicability in the cosmetics industry from the industrial byproduct of A. sisalana. Initially, the sisal byproduct was collected and subsequently dried. Two different extraction methods were performed, using a Soxhlet apparatus and pressurized liquid resulting in eighteen extracts, nine from each extraction method. Total tannins and diosgenin quantification were performed on the extracts that showed the highest crude yield after extraction and drying. The extract obtained by Soxhlet was chosen for the following evaluations, which was prepared using 30 % ethanol in 8 hours of extraction and had a crude yield of 8.07 %. In this extract, tannins, flavonoids and saponins were identified. In the in vivo safety assessment, the extract shown to be safe for use on human skin. In the in vitro evaluation of antioxidant activity, the extract showed DPPH radical scavenging activity (IC50 = 1.89 mg/mL) and ABTS•+ radical scavenging activity (IC50 = 0.37 mg/mL). Total antioxidant capacity was 18.55±0.33 Eq.mg of ascorbic acid/g of extract, in addition to presenting the capacity to chelate Cu2+ and Fe2+ and discrete OHradical scavenging capacity. In the antityrosinase activity evaluation, the extract presented 10.41±2.23 % of inhibition in the concentration of 10 mg/mL. In this context, the use of the A. sisalana by-product to develop a new multifunctional bioingredient, with antioxidant and skin lightener, for the cosmetics industry can contribute to minimizing environmental impacts and to the socioeconomic development of regions that produce sisal fibers.pt-BRAcesso AbertoAgave (Agave sisalana)SisalAntioxidanteAntitirosinaseCosméticosdoctoralThesisCIENCIAS DA SAUDE