Farias, Naisandra Bezerra da SilvaBarichello, Priscila Arend2017-05-122017-05-122015-06-29BARICHELLO, Priscila Arend. Alterações morfológicas e expressão de VEGF, TGFα, FGF2 e FGF7 na pele sob o efeito da radiofrequência associada ao uso da própolis. 2015. 62f. Dissertação (Mestrado em Biologia Estrutural e Funcional) - Centro de Biociências, Universidade Federal do Rio Grande do Norte, Natal, 2015.https://repositorio.ufrn.br/jspui/handle/123456789/22894When an electromagnetic wave is emitted in the frequency range between 3 KHz to 24 GHz, interacts with the skin, promoting the diathermy, i.e. the heat through deep heat, converting electrical energy into thermal energy. Dermato-functional treatment is non-invasive, which leads to better supply of nutrients, circulatory and tissue hydration, increasing oxygenation, acceleration of the elimination of catabolites and contraction of the connective tissue. Promotes reorientation of collagen fibers and its increase in thickness as well as epithelial tissue. Propolis is a resinous substance from the european honeybee Apis Mellifera harvested buds, flowers and plant exudates and has antioxidativos effects that promote the synthesis of collagen. This aim seeks to investigate the effects of propolis associated with radiofrequency, the morphology of the epidermis and dermis of Wistar rats. So, waiting for a positive action on neocolagenogênese. The methodology used was composed of 36 Wistar rats, weighing between 250 to 300. Divided into 4 groups, containing 9 animals in each group going on 3 radiofrequency applications and/or propolis in three weeks: G1 – control (C); G2-Propolis (P); G3 – Radiofrequency + Propolis (RP); G4-Radiofrequency (R). After the end of treatment, 24 hours the animals were eutanasiados and subsequently collected fragments of skin that were fixed in 10% paraformoldehyde and subjected to routine histological techniques (dehydration, diafanização and inclusion in paraffin), microseccionados to 5 μm and stained with Hematoxylin and Eosin, Picrossírius-Red and immunolabeling for TGFα, FGF2, FGF7 and VEGF. For analysis and comparisons of the epidermis, papillary and reticular dermis between groups, and the weight and morphology of the collagen fibers, microphotographs were obtained and microprocessadas by the ImageJ Software 1.49 J. Our results reveal that the epidermis of the propolis group was the most developed, as well as the markup for the FGF7. The total collagen was more expressive in all groups that have suffered treatment, however the papillary and reticular dermis appeared lower in the groups treated with RF, possibly due to collagen contraction after application. It was observed that the higher expression in FGF2 radiofrequency groups with propolis and radiofrequency. The largest imunopositivity in the group showed TGFα with propolis, however this group showed no granulation tissue. The VEGF showed no significant difference between the groups. No group presented swelling or inflammation. We conclude that collagen formation occurs in all treated groups, and propolis acts as an activator of keratinocyte proliferation. Thus, our findings are of the utmost importance, as it will help to increase new technologies related to dermal neocolagenogênese mechanisms, key issue for new possibilities of application on tissue engineering and dermato-functional area.Acesso AbertoEpidermeDermeFibras colágenasMorfologiaAlterações morfológicas e expressão de VEGF, TGFα, FGF2 e FGF7 na pele sob o efeito da radiofrequência associada ao uso da própolismasterThesisCNPQ::CIENCIAS BIOLOGICAS: BIOLOGIA ESTRUTURAL E FUNCIONAL