Pinto, Leão PereiraRolim, Larissa Santos Amaral2018-06-112018-06-112018-02-23ROLIM, Larissa Santos Amaral. Análise comparativa da imunoexpressão de twist e da podoplanina entre carcinomas de células escamosas de língua oral e de lábio inferior. 2018. 102f. Dissertação (Mestrado em Patologia Oral) - Centro de Ciências da Saúde, Universidade Federal do Rio Grande do Norte, Natal, 2018.https://repositorio.ufrn.br/jspui/handle/123456789/25326Introduction: Oral Squamous Cell Carcinoma (OSCC) is a major public health problem worldwide. It is believed that the mechanism responsible for tumor progression is based on collective invasion of cell groups or individual cells, called Epithelial-Mesenchymal Transition (EMT), where there is a decrease in the expression of epithelial biomarkers and increased expression of mesenchymal biomarkers as Twist. Podoplanin (PDPN), a transmembrane glycoprotein, is assumed to be involved in the motility of neoplastic cells that are undergoing through EMT to guide the cell complex during an invasion. Objective: To perform a comparative analysis of the immunoexpression of PDPN and Twist among 40 cases of lower lip CCEs and 36 cases of oral tongue, (primary tumor size, regional and distal lymph node metastasis, clinical staging, histological grade of malignancy and histological pattern of invasion). Methods: To evaluate the histopathological grade of malignancy, the system proposed by Brandwein-Gensler et al. (2005) was used. For the two markers, four types of immunohistochemical analysis were performed: analysis of the invasion front, tumor compressive areas, large tumor islands (> 15 neoplastic cells) and nests/dissociated cells (<15 neoplastic cells). The non-parametric Mann-Whitney test was used for the analysis of the relationships between the clinical and histopathological parameters and the PDPN and Twist immunoexpressions. To verify possible correlations between the PDPN and Twist immunoexpressions, the Spearman (r) correlation test was performed. For all statistical tests, a significance level of 5% (p <0.05) was established. Results: No statistically significant differences were found between PDPN and Twist immunoexpression and tumor size, metastasis, and clinical staging on lower lip SCC (p > 0.05). However, statistically significant differences were found between the cytoplasmic immunoexpression of PDPN and the invasion patterns type 4 and 5 (p = 0.032) of Brandwein-Gensler et al. (2005). Also, no statistically significant differences were found between PDPN and Twist immunoexpression and tumor size, metastasis and clinical staging on oral tongue SCC (p > 0.05). Statistically significant differences were observed between the cytoplasmic (p = 0.006), membrane (p = 0.030) and general (p = 0.025) PDPN expression in oral tongue SCC with the invasion patterns type 4 and 5 of Brandwein-Gensler et al. (2005). In addition, the Spearman correlation test demonstrated statistically significant negative correlations between PDPN membrane expression and Twist general expression (r = -0.356, p = 0.024) and Twist cytoplasmic expression (r = -0.336; p = 0.034) in the lower lip SCCs. Conclusions: PDPN expression is inversely related to Twist in lower lip SCC, it was demonstrated that both proteins are associatved with a worse invasion pattern in SCC. It has also been hypothesized that the relationship of PDPN with Twist in OSCC may be more involved with a partial transition from the epithelial to the mesenchymal phenotype than a complete transition coordinated by PDPN.Acesso AbertoCarcinoma de células escamosasProteína 1 relacionada a twistTransição epitelial-mesenquimalPatologia bucalmasterThesisCNPQ::CIENCIAS DA SAUDE: PATOLOGIA ORAL