Andrade, Vânia SousaFreitas, Laryssa da Silva2025-07-182025-07-182025-07-08FREITAS, Laryssa da Silva. Avaliação in vitro da suscetibilidade a antifúngicos de isolados de leveduras de corrente sanguínea em pacientes internados no Hospital Universitário Onofre Lopes, Natal/RN. Orientadora: Vânia Sousa Andrade. 2025. 67 f. Monografia (Graduação em Biomedicina) – Departamento de Microbiologia e Parasitologia, Universidade Federal do Rio Grande do Norte, Natal, 2025.https://repositorio.ufrn.br/handle/123456789/64669Candidemia is an invasive fungal infection caused by species of the genus Candida, which affects the bloodstream and represents one of the main causes of morbidity and mortality in hospital settings. This study aimed to evaluate the antifungal susceptibility profile of Candida isolates obtained from blood cultures of patients hospitalized at Onofre Lopes University Hospital (HUOL), in Natal/RN, from December 2023 to November 2024. Species identification was performed through automated biochemical methods, chromogenic media, and MALDI-TOF mass spectrometry. Antifungal susceptibility was determined by minimum inhibitory concentration (MIC). A high concordance rate was observed between identification methods (96.87%), with a predominance of the Candida parapsilosis complex among the isolates (46.88%). No resistance was detected to amphotericin B, micafungin, or caspofungin. However, resistance to fluconazole was observed in two strains of Nakaseomyces glabratus and one of Candida tropicalis; one Candida albicans strain was resistant to itraconazole, two C. albicans to voriconazole, three C. albicans to posaconazole, and one C. albicans to anidulafungin. The predominance of C. parapsilosis observed in this study confirms the trend described in the literature regarding the increase of non-albicans Candida species in candidemia cases. Antifungal resistance in Candida spp. may be favored by prolonged antifungal use. In the present study, a high susceptibility to micafungin (100%) was observed, while fluconazole showed lower susceptibility, especially in C. tropicalis (75%) and N. glabratus (33.3%). These findings align with international trends and highlight the reduced efficacy of fluconazole in certain species, as well as the low occurrence of resistance to echinocandins and amphotericin B, supporting data from the global literature. Therefore, the importance of continuous microbiological surveillance and rational use of antifungals is emphasized as essential strategies for controlling candidemia in hospital settings.pt-BRAttribution 3.0 Brazilhttp://creativecommons.org/licenses/by/3.0/br/FungemiaMicrodiluição em caldoMicoses oportunistasDisseminação hematogênicaBroth microdilutionOpportunistic mycosesHematogenous disseminationbachelorThesis