Ferreira, Leonardo CapistranoHolanda, Ingrid de Paula2019-07-022021-10-062019-07-022021-10-062019-06-07HOLANDA, Ingrid de Paula. Análise da expressão gênica das aminopeptidases ERAP1, ERAP2 e LNPEP em gestantes com pré-eclâmpsia. 2019. 52f. Trabalho de Conclusão de Curso (Graduação em Biomedicina) - Centro de Biociências, Universidade Federal do Rio Grande do Norte, Natal, 2019.https://repositorio.ufrn.br/handle/123456789/43209Pre-eclampsia is a hypertensive disease of pregnancy characterized by the development of hypertension after the 20th week of pregnancy accompanied by proteinuria. It is the major cause of maternal-fetal death and is highly complex due to its multifactorial nature. Genetic studies in different populations indicated the ERAP1, ERAP2 and LNPEP genes as important candidate genes for pré-eclâmpsia. These genes encode aminopeptidases, enzymes involved in various biological processes ranging from cancer to infections, in addition to hypertension. Through its peptidase activity, ERAP1 and ERAP2 regulate blood pressure (target: angiotensin II) and LNPEP promotes homeostasis in pregnancy and delivery (target: oxytocin). The ERAP1 gene has variants associated with autoimmune diseases and essential hypertension, they are rs30187 and rs27044, these variants cause functional alterations in the gene product. OBJECTIVE: The objective of this study was to evaluate the gene expression of aminopeptidases in pregnant women with pre-eclampsia (n = 25) and normotensive (n = 21), and to evaluate the effect of ERAP1 variants (rs30187 and rs27044) on the expression of aminopeptidases in different groups. METHODS: Gene expression of aminopeptidases was evaluated using the real-time PCR technique and the variants were genotyped using the SNaPshot® technique. The tests were performed from whole blood samples of pregnant volunteers from the Maternidade Escola Januário Cicco. RESULTS: The pre-eclampsia and normotensive groups had distinct aminopeptidase expression profiles (p = 0.01). Variants rs30187 and rs27044 had no effect on ERAP1 expression levels (p-value> 0.05). The plasma concentration of Erap1 in the analyzed samples was below the detection limit of the assay (0.156 ng / ml). CONCLUSION: The study showed differences in the aminopeptidase expression profile between the groups, highlighting the involvement of these aminopeptidases with the hypertensive diseases of pregnancy that has been evidenced in recent times in the literature.Pré-eclâmpsiaAminopeptidasesGenéticaSNPbachelorThesis