Araújo, Joselio Maria Galvão deMedeiros, Leandro Gurgel de2018-05-142018-05-142018-02-19MEDEIROS, Leandro Gurgel de. Pesquisa dos vírus dengue em culicídeos no Município de Natal, Rio Grande do Norte, 2016. 2018. 104f. Dissertação (Mestrado em Biologia Parasitária) - Centro de Biociências, Universidade Federal do Rio Grande do Norte, Natal, 2018.https://repositorio.ufrn.br/jspui/handle/123456789/25210Dengue is a complex and multifactorial disease, found mainly in tropical and subtropical regions, with a global incidence ranging from 50 to 100 million cases per year. Its etiologic agent is dengue virus (DENV), a group B arbovirus, so designated because its maintenance in nature depends on transmission to vertebrate hosts by hematophagous arthropod vectors (Artrophod-Borne Virus). They are transmitted to humans by female mosquitoes of the genus Aedes, especially Aedes aegypti and Aedes albopictus. DENVs are single-stranded RNA viruses with positive polarity, approximate size 11Kb and with a single open reading frame. It is classified in the Flaviviridae family, genus Flavivirus, and four serotypes (DENV-1, DENV-2, DENV-3 and DENV-4) are described, serologically similar, but with specific antigenic determinants for each serotype. This work aimed to investigate the dengue virus in culicidae captured in the municipality of Natal-RN, Brazil, from January to November, 2016. We analyzed 137 pools of females of the species Aedes aegypti, Aedes albopictus, Culex quinquefasciatus, Wyeomyia sp., Ochlerotatus scapularis and Haemagogus leucocelaenus. Each pool was analyzed for the genetic material of DENV by the RT-PCR technique. The species Aedes aegypti was the most represented, with 72 analyzed pools. RNA was detected in 19,70% (27/137) of the pools. In the western and northern sanitary districts, there was a higher incidence of mosquitoes collected. Two types of serotypes were detected: DENV-1 and DENV-3. DENV-1, the most prevalent serotype, was detected in 92,60% of the positive pools (25/27), while DENV- 3 was detected in only 7,40% (2/27). The months of February and April were the ones that had a greater number of mosquito pools tested positive for DENV, both with 44,4% (12/27) of the total positive pools. This information allowed the recognition of priority areas for dengue control actions in Natal-RN, as well as the serotypes circulating in the municipality during the year of the study and revealed the presence of DENV in other species of culicidae, a pioneer finding in the municipality.Acesso AbertoMosquitosVetores de dengueArbovírusDistritos sanitáriosmasterThesisCNPQ::CIENCIAS BIOLOGICAS: BIOLOGIA PARASITÁRIA