Giordani, Raquel BrandtReginaldo, Fernanda Priscila Santos2021-07-212021-07-212021-03-25REGINALDO, Fernanda Priscila Santos. Selaginella convoluta (Arn.) Spring: uma abordagem fitoquímica aliada a estudos ômicos. 2021. 91f. Tese (Doutorado em Desenvolvimento e Inovação Tecnológica em Medicamentos) - Centro de Ciências da Saúde, Universidade Federal do Rio Grande do Norte, Natal, 2021.https://repositorio.ufrn.br/handle/123456789/32918The genus Selaginella, the largest genus of Lycopodiophyta comprise about 700- 750 species, which several species are recognized for their pharmacological activities. Our overview of the phytochemical and bioactive aspects pointed out that future studies should afford valuable new data on better explore the biological potential of the flavonoid amentoflavone and their derivatives as chemical bioactive entities; develop studies about toxicity and, finally, concentrate efforts on elucidate mechanisms of action for biological properties already reported. The family Selaginellaceae managed to survive the many biotic and abiotic pressures during the last 400 million years. Several species of the genus Selaginella have been acquired an interesting evolutionary trait: desiccation tolerance (DT). Selaginella convoluta is a desiccation-tolerant plant native from Brazilian semiarid. DT plants have highly effective drought-resistance mechanisms that allow their survival under extreme conditions. In this study we investigate Selaginella convoluta in two hydration states: (i) dehydrated – harvested in the natural semiarid environment; and (ii) hydrated – after acclimatization in lab. The proteome dataset pointed out that shoots and roots appear to have different strategies in response to different hydration states; however, appear to have a well-orchestrated response to regulate desiccation tolerance. The presence of enzymes involved in the biosynthesis of specialized metabolites such as phenolic, terpenoid, as well as metabolites containing nitrogen/sulfur were identified through proteomic analysis. The NMR-based metabolite profiling showed the presence of metabolites such as carbohydrates and phenolic derivatives related to desiccation tolerance events, corroborating with the proteome data. As an unprecedented finding, the most important annotated alkaloid was anabasine. The specialized metabolites, flavonoids, bioflavonoids and selaginellins were the main phenolic derivatives annotated. Thus, we performed a LC-MS/MS analysis combined with the molecular networking (MN) approach to assess the chemodiversity of flavonoids and selaginellins. So far, this has been the first study on Selaginella spp. using the MN approach that showed not only a proposal of the fragmentation pattern for all the selaginellins annotated, but also the prediction of new selaginellins based on the MS/MS spectral data. Several biological potentials of selaginellin from Selaginella spp. have been reported. Selaginellins are naturally produced at low levels in the plant, which makes the chemical extraction of these metabolites difficult and time consuming. In this context, methyl jasmonate (MeJA) has been used to induce specialized metabolites of pharmaceutical interest. Our data showed that MeJA can be an interesting alternative to induce the accumulation of selaginellins in S. convoluta. In addition, the in-silico studies pointed out interesting bioactive potentials for selaginellins: antineurodegenerative, antiproliferative and antiparasitic. The MN approach allowed the prediction of seven unprecedent selaginellins, which contribute as a new chemical information for this species.Acesso AbertoSelaginelinasAnabasinaProteomaMetabolomaTolerância à dessecaçãoMetiljasmonatodoctoralThesis