Freitas, Janaína Cristiana de Oliveira CrispimLima, Maysa Eunice Grigorio Bezerra2023-02-162023-02-162022-12-13LIMA, Maysa Eunice Grigorio Bezerra. Estudo do polimorfismo de 14 pb do Gene HLA-G em mulheres com infertilidade. 2022. 40 f. Trabalho de Conclusão de Curso (Graduação em Biomedicina) – Centro de Biociências, Universidade Federal do Rio Grande do Norte, Natal, 2022.https://repositorio.ufrn.br/handle/123456789/51308Human Leukocyte Antigen - G (HLA-G) is involved in the pregnancy process and modulates the implantation of the semi-halogenous embryo from processes involving immune tolerance at the maternal-fetal interface. The objective of the present study was to evaluate the 14 bp INS/DEL polymorphism of the 3' untranslated region (3' UTR) that is located in exon 8 of the HLA-G gene in women with infertility undergoing in vitro fertilization (IVF). Patients undergoing IVF were selected to undergo venepuncture for genomic DNA extraction and 14bp gene polymorphism of the HLA-G gene. The blood was obtained from 35 women diagnosed with infertility treated at the Assisted Reproduction Center of the Januário Cicco Maternity School. All women surveyed signed the Informed Consent Form (ICF). The method for amplification of the polymorphism was nested-PCR on a 3% agarose gel. For both SNPs, the PCR conditions were standardized using the MyCycler™ Thermal Cycler (Bio-rad, USA). Regarding heterozygous genotype (INS/DEL), the data showed that patients who had infertility had a higher frequency of these genotypes when compared to healthy controls, however these data were not statistically significant (P>0.05). Regarding the ins/del alleles (rs66554220) no association was also found between the 14bp gene of the HLA-G gene in women with infertility when compared to the healthy control.HLA-GImunologia reprodutivaReprodução HumanaHLA-GHuman ReproductionReproductive ImmunologybachelorThesis