Costa, Leandro SilvaNegreiros, Marília Medeiros Fernandes de2016-04-282016-04-282015-08-07NEGREIROS, Marília Medeiros Fernandes de. Nanopartículas de prata contendo polissacarídeos sulfatados de algas: caracterização química, morfológica e identificação de suas atividades antioxidante, bactericida, antiproliferativa e imunomodulatória. 2015. 138f. Dissertação (Mestrado em Bioquímica) - Centro de Biociências, Universidade Federal do Rio Grande do Norte, Natal, 2015.https://repositorio.ufrn.br/jspui/handle/123456789/20331The production of silver nanoparticle containing natural extracts has been identified as an excellent alternative to enhance or provide new applicability for these extracts. Sulfated polysaccharides-rich extracts (ASP) from seaweeds showed several pharmacological properties, but there are few reports of the production of silver nanoparticle with sulfated polysaccharides (SPN). Therefore, this paper we synthesized SPN from seaweeds founded in Brazil: Spatoglossum schröederi, Dictyopteris justii, Sargassum filipendula e Dictyota mertensii. The obtainment of polysaccharides rich extracts it occurred by proteolysis followed by methanol precipitation. The synthesis of various nanoparticles occurred with the addition of silver solution 1 mM on different polysaccharides solutions and kept at rest. Subsequently the samples were centrifuge and lyophilized. The SPN formation was confirmed by UV/ visible spectroscopy, scanning electron microscopy and atomic force microscopy. The size of SPN was 108 ± 2 nm; 82 ± 1nm; 288 ± 52 nm; 104 ± 2 nm for S. schröederi; D. justii; S. filipendula; D. mertensii, respectively. The nanoparticules remained stable for fourteen months, the Zeta potential were negative and forms of SPN were rounded. Data from various antioxidant in vitro tests showed that the SPN were more effective than ASP. The SPN were also biocompatible with the normal cells 3T3 (murine fibroblast). Moreover, SPN of S. schröederi and of D. mertensii had cytotoxic activity (~60%) against melanoma cells (B16F10). We believe that the increased of cytotoxic activity of these SPN occur due to their small size. SPN also had great antibacterial capacity. D. justii and S. filipendula nanoparticles had the best results, requiring only 50 mg/mL and 100 mg/mL for induces E. coli and S. aureus death. We hypothesized that this activity occurs by release of SPN’s silver. SPN also increase the nitric oxide and cytokines release from macrophages as similar profile to the respective ASP, except for S. schröederi SPN. In general, the results showed that SPN were more effective as antioxidant, antibacterial and cytotoxic agents than their respective ASP, as well as, SPN have shown the same immunomodulation effect of the respective ASP. The results lead to propose that the SPN synthesis constituted as a possible potentiating mechanism of biological activities of ASPs.porAcesso AbertoNanotecnologiaHeterofucanasAlgas marronsmasterThesisCNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA