Gasparotto, Luiz Henrique da Silvahttp://lattes.cnpq.br/6928918856031880Silva, Heloiza Fernanda Oliveira da2016-05-122016-05-122015-07-20SILVA, Heloiza Fernanda Oliveira da. Nanopartículas de prata conjugadas com hiclato de doxiciclina: síntese, caracterização e avaliação da atividade antimicrobiana contra Escherichia coli. 2015. 90f. Dissertação (Mestrado em Química) - Centro de Ciências Exatas e da Terra, Universidade Federal do Rio Grande do Norte, Natal, 2015.https://repositorio.ufrn.br/jspui/handle/123456789/20435Silver nanoparticles, AgNPs have been used to combat bacterial resistance. The approach for associate the drug to stabilizing agent is completely new. It is focus area on nanometerials necessity of removing the particle stabilizing agent because it consumes other sites active for adsorption. However, in the present study shows that the presence of the stabilizing agent is extremely beneficial as observed in antimicrobial results. The goal was to combine Doxycycline hyclate to NPsAg and evaluate the antimicrobial effect. The spectroscopy in the UV-Vis region showed bathochromic shift in the absorption band of free NPsAg for NPsAg-HDOX indicating the possible existence of different species from the others. The Fluorescence Spectroscopy provided the information that the HDOX acted as a suppressor of issuing NPsAg, from these data we determined the Stern-Volmer relationship that indicated the presence of HDOX in the sphere of action, it is very close to the particle. The Spectroscopy in Time Resolved Fluorescence HDOX showed that has no interacted with the surface of the particle. The Spectroscopy Infrared Attenuated Total Reflectance, FTIR-ATR showed a shift of the amide carbonyl stretching band of 1680 cm-1to 1672 cm-1, showing that there is an interaction between the HDOX and NPsAg stabilized by PVP. The scattering curves in small angle x-ray scattering, SAXS showed that within the first 3 hours after addition of the HDOX NPsAg. In the other hand, Transmission Electron Microscopy indicated that after 72 hours the particles suffer na increase in distribution size. Thus providing a basis regarding the aging combined. The assessment of the antimicrobial effect was performed using the diffusion method. The inoculum used was standard strains of Escherichia coli. The results indicated that all NPsAg-HDOX combined had antimicrobial effect which was greater than the free HDOX (95% confidence level).porAcesso AbertoNanopartículas de prataHiclato de doxiciclinaEfeito antimicrobianomasterThesisCNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA