Use of polyethylenimine to produce immobilized lipase multilayers biocatalysts with very high volumetric activity using octyl-agarose beads: Avoiding enzyme release during multilayer production

Peña, Sara AranaRios, Nathalia SaraivaSanchez, Carmen MendezLokha, YuliyaGonçalves, Luciana Rocha BarrosLafuente, Roberto Fernández2021-11-292020-06ARANA-PEÑA, Sara; RIOS, Nathalia S.; MENDEZ-SANCHEZ, Carmen; LOKHA, Yuliya; GONÇALVES, Luciana R.B.; FERNÁNDEZ-LAFUENTE, Roberto. Use of polyethylenimine to produce immobilized lipase multilayers biocatalysts with very high volumetric activity using octyl-agarose beads: avoiding enzyme release during multilayer production. Enzyme And Microbial Technology, [S.L.], v. 137, p. 109535, jun. 2020. Disponível em: https://www.sciencedirect.com/science/article/abs/pii/S0141022920300284?via%3Dihub#!. Acesso em: 29 nov. 2021. https://doi.org/10.1016/j.enzmictec.2020.1095350141-0229https://repositorio.ufrn.br/handle/123456789/45067Ion exchangeIntermolecular and interlayer crosslinkingImmobilization of lipasesSubstrate diffusion problemsEnzyme releaseUse of polyethylenimine to produce immobilized lipase multilayers biocatalysts with very high volumetric activity using octyl-agarose beads: Avoiding enzyme release during multilayer productionarticle10.1016/j.enzmictec.2020.109535