Agnez, Lucymara FassarellaLeal, Angélica Maria de Sousa2017-04-202016-09-29LEAL, Angélica Maria de Sousa. O papel da via de reparo por excisão de nucleotídeos na resposta celular ao estresse oxidativo e o estudo de alterações neuronais in vitro associadas a síndrome de Cockayne. 2016. 167f. Tese (Doutorado em Bioquímica) - Centro de Biociências, Universidade Federal do Rio Grande do Norte, Natal, 2016.https://repositorio.ufrn.br/jspui/handle/123456789/22713In oxidative stress response, the base excision repair (BER) is considered the major pathway for repair of oxidative lesions. However, an increasing number of studies have indicated the role of nucleotide excision (NER) in the repair of these lesions. In addition, some NER factors had functions beyond the role in repair already described and it is important to search for new molecular functions that can be associated to the classical phenotypes of the syndromes caused by mutations in NER genes: Xeroderma pigmentosum complementation group A, caused by mutations in XPA and Cockayne syndrome, caused by mutations in CSB. In this context, XPA (XP12RO) or CSB (CS1AN) deficient cells were submitted to oxidative stress induced by Hydrogen peroxide (H2O2) and the results indicated that both cell lines showed sensitivity to this agent. Furthermore, the transcriptome of XP12RO cells revealed the downregulation of genes that play a role in DNA damage response and promote cell survival in response to oxidative stress. In this scenario, the results indicated that XPA regulates the expression of genes that play a key role in DNA damage response and promote survival in response to stress (EGR1, GADD45A, GADD45B and XPC). On the other hand, the transcriptome analysis of CS1AN cells showed the downregulation of genes that play a key role in biological processes such as transcription, mRNA processing, protein degradation by the ubiquitin–proteasome pathway proteolysis or cellular respiration, indicating a possible role for CSB protein in the regulation of these processes, in response to oxidative stress. In adittion, given the neurodegeneration phenotype associated to Cockayne syndrome, neural progenitor cells (NPCs) and neurons derived from CSB deficient induced pluripotent stem cells (iPSCs) were used as cellular models to analyse neuronal changes in vitro. The results showed that, as observed in fibroblasts CS1AN, NPCs also presented sensitivity to oxidizing agents. Furthermore, as indicated in the transcriptome data from CS1AN fibroblasts, given the downregulation of genes that play a pivotal role in cellular respiration, the analysis of oxygen consumption rates in CSB deficient neurons also indicated a mitochondrial dysfunction characterized by the decrease in oxygen consumption basal rate and a lower maximum respiratory and reserve capacities, suggesting that the lack of functional CSB leads to a mitochondrial dysfunction in both cellular models used in this study.Acesso AbertoEstresse oxidativoReparo por excisão de bases (BER)Reparo por excisão de nucleotídeos (NER)XPACSBSíndrome de CockayneCélulas-tronco pluripotentes induzidas (iPSCs)NPCsNeurôniosRespiração celulardoctoralThesisCNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA