Balaban, Rosângela de CarvalhoCâmara, Paulo César França da2021-08-182021-08-182021-02-02CÂMARA, Paulo César França da. Obtenção de biomateriais utilizando derivados de taninos e glicosaminoglicanos para aplicações na engenharia tecidual e medicina regenerativa. 2021. 95f. Tese (Doutorado em Química) - Centro de Ciências Exatas e da Terra, Universidade Federal do Rio Grande do Norte, Natal, 2021.https://repositorio.ufrn.br/handle/123456789/33209Developing stable polysaccharide structures with biochemical and biomechanical properties similar to the extracellular matrix (MEC) is one of the major goals of tissue engineering and regenerative medicine. Biomaterials used in medical devices need to be compatible with different biological media, without causing adverse reactions such as rejection and failure. Increasing the biocompatibility of conventional materials through surface modification has been shown to be an effective method for improving interaction with cells and blood components, for example. This work reports a new class of surface coatings for the purpose of enhancing biocompatibility and cellular response using multilayer self-assembled polyelectrolytes (PEMs) with a derivative of cationic tannin (tanfloc) and glycosaminoglycans (chondroitin sulfate or heparin) because they exhibit similar chemical structures to the ones found in the ECM. The construction of polyelectrolyte multilayers was monitored by Fourier Transform Surface Plasmon Resonance (FT-SPR). The surface properties were assessed by X-ray Excited Photoelectron Spectroscopy (XPS), Atomic Force Spectroscopy (AFM) and contact angle measurements. The response of human adipose-derived stem cells (ADSCs) was evaluated in vitro through cytocompatibility assays, adhesion and proliferation of the cells using fluorescence microscopy. For the hemocompatibility assays, the adsorption of key serum proteins, adhesion and platelet activation, as well as whole blood coagulation assays were monitored by XPS, scanning electron microscopy (SEM) and visible ultraviolet (UV-vis) spectroscopy. The interactions of ADSCs with these new biomaterial surfaces demonstrate that these surface coatings exhibit good cytocompatibility and that they promote cell attachment and proliferation. Blood tests point to the high potential of developing hemocompatible materials through tannin derivatives. Particularities in the chemical structure of tanfloc were signaled as crucial in the control of blood protein adsorption, such as fibrinogen, creating an antiplatelet effect on the material’s surface. The association between biomaterial components, cells and key bioactive molecules in biological processes important in the reconstruction of large tissue losses highlights the great potential of materials. These characteristics are very desirable for biocompatible coatings for applications such as tissue engineering scaffolds and medical devices.Acesso AbertoMulticamadas de polieletrólitosGlicosaminoglicanosTaninosBiomateriaisCélulas-troncodoctoralThesis