Scortecci, Katia CastanhoSilva, Ariana Pereira da2024-08-162024-08-162023-12-22SILVA, Ariana Pereira da. Prospecção da atividade antioxidante in vitro e in vivo dos extratos da semente de chañar (Geoffroea decorticans) (Gill. ex Hook. et Arn.) Burkart (Fabaceae). Orientadora: Dra. Kátia Castanho Scortecci . 2023. 83f. Dissertação (Mestrado em Bioquímica e Biologia Molecular) - Centro de Biociências, Universidade Federal do Rio Grande do Norte, Natal, 2023.https://repositorio.ufrn.br/handle/123456789/59299The use of plants for medicinal purposes is an ancient practice, characterized by the use of various parts of these organisms as complementary therapeutic resources. Geoffroea decorticans, popularly known as Chañar, is a plant native to Chile, belonging to the Fabaceae family. All parts of this plant have uses in folk medicine, ranging from expectorant activity, including bronchopulmonary disorders and pain relief, as well as antioxidant and antinociceptive activity. However, few studies are reporting such activities with extracts of Chañar seed. That said, our work aimed to evaluate and characterize the antioxidant, cytotoxic and protective potential against oxidative stress induced by copper sulfate (CuSO4) of the ethanolic (EE) and aqueous (EA) extracts of Chañar seed using in vitro and in vivo methodologies. The two extracts produced were phytochemically characterized using HPLC - GC-MS/MS, as well as spectrophotometric tests such as total phenolic compounds and total flavonoids. Subsequently, biochemical tests were carried out to assess the in vitro antioxidant potential using five spectrophotometric tests (total antioxidant capacity-CAT, reducing power; 2,2-diphenyl-1-picrylhydrazyl-DPPH; copper and iron chelation) aimed at verifying the possible modes of action by which these bioactive compounds could be contributing to the maintenance of cellular redox homeostasis. The 3T3 ATCCCCL-92 fibroblast cell line was also used to assess the cytotoxicity of EE and EA, as well as the protective role of the extracts when this cell line was subjected to the stress agent copper sulfate (CuSO4), in addition to the potential to induce cell migration or not. After the in vivo tests, the extracts were tested on the invertebrate animal model Tenebrio molitor. Survival tests were carried out on this model to assess the toxicity of the extracts and their protective capacity against oxidative stress caused by copper sulfate, as well as melanization parameters. The results obtained by chromatography detected the presence of secondary metabolites such as phytol, alpha-tocopherol, vitexin and rutin. The in vitro spectrophotometric tests demonstrated the antioxidant capacity of the extracts to act by different mechanisms of action (inhibiting, sequestering and chelating). Meanwhile, tests on the 3T3 cell model demonstrated the absence of EE and EA cytotoxicity, as well as protection against (CuSO4) stress and cell migration capacity. In the animal model, the extracts once again showed their excellent protective capacity against oxidative stress, capable of combating the free radicals generated, mitigating the melanization exacerbated. Taken together, the results of the tests (in vitro and in vivo) show that the EE and EA extracts of Chañar seeds are a source of bioactive compounds with excellent antioxidant properties, which also strengthens the use of the derivatives generated by the plant, given that depending on the consumption of the fruit, the seeds are considered to be crop waste.Acesso AbertoExtrato etanólicoExtrato aquosoCompostos fenólicosEstresse oxidativoSulfato de cobreTenebrio molitormasterThesisCNPQ::CIENCIAS BIOLOGICAS