Araújo, Joselio Maria Galvão deMonteiro, Joelma Dantas2016-09-062016-09-062016-01-26MONTEIRO, Joelma Dantas. Epidemiologia molecular dos vírus dengue e zika no estado do Rio Grande do Norte, no período de junho de 2014 a maio de 2015. 2016. 110f. Dissertação (Mestrado em Biologia Parasitária) - Centro de Biociências, Universidade Federal do Rio Grande do Norte, Natal, 2016.https://repositorio.ufrn.br/jspui/handle/123456789/21368The etiological agents of dengue fever and zika are single-stranded RNA viruses with positive polarity belonging to the Flaviviridae family, genus flavivirus and is transmitted to humans through the bite of blood-sucking arthropod vectors of the genus Aedes. The state of Rio Grande do Norte (RN) lives with dengue cases for over two decades. Regarding the zika fever, until the first day of May 2015, the Ministry of Health had not confirmed any cases of the disease in Brazil, either in RN, although already had clinical suspicion of infection caused by this virus. This study aimed to analyze the epidemiological profile of Dengue and Zika virus in the state of Rio Grande do Norte, in the period June 2014 to May 2015. A total of 334 blood samples from patients were analyzed for dengue virus (DENV) by RT-PCR technique where 8.08% were positive and 348 samples which were analyzed to detection Zika vírus (ZIKV) by means of qRT-PCR, of which 20.98% were positive. It was found the movement of DENV serotypes-1, DENV-2 and DENV-4 in infants in the study period, with a predominance of this, detected in 66.67% (18/27) of positive cases. The municipalities of Natal, Jandaíra and Ouro Branco had a higher number of confirmed cases to the DENV, with 22.22% (6/27), 18.52% (5/27) and 11.11% (3/27), respectively. With regard to the ZIKV, the cities with highest numbers of confirmed cases were Natal, Parnamirim, Guamaré e Nova Cruz with 30.14% (22/73), 19.18% (14/73), 13.70% (10/73) and 9, 59% (7/73) respectively. The first town to have a confirmed case of DENV during the study period was Jandaíra. Caiçara of Rio of Vento was the first to present a case ZIKV, followed by Galinhos. June was the most representative month in the number of confirmed cases of DENV with 44.44% (12/27), while March was the month with the highest number of confirmed cases of ZIKV with 23.29% (17/73). The male and females have about the same proportion of detected cases for DENV, with 51.85% (14/27) and 48.15% (13/27) respectively, while the ZIKV occurred in a larger number of women, representing 57.53% (42/73) of cases. The age groups most affected by dengue were 11-20 and 51-60 years, each with 18.52% (5/27) each one. Regarding the ZIKV, the age of 0-10 years was the most frequent with 19.18% (14/73) of the confirmed cases, followed by ages 31-40 and 41-50 years, where each of these represented 16 44% (12/73). The comparative analysis of the viral load between 5 days of fever zika performed by qRT-PCR showed a viral load higher on the 3rd day of the disease. Once proven the presence of these two flavivirus in RN, it is necessary to know the prevalence and circulation dynamics of each of the local population in order to establish control measures aimed at avoiding future outbreaks and epidemics in the state. This study represents the largest study on the virus Zika in the state of RN.Acesso AbertoDengueZikaEpidemiologiaRio Grande do NorteEpidemiologia molecular dos vírus dengue e zika no estado do Rio Grande do Norte, no período de junho de 2014 a maio de 2015masterThesisCNPQ::CIENCIAS BIOLOGICAS: BIOLOGIA PARASITÁRIA