Santos, Everaldo Silvino dosSantos, Davi Alves dos2017-12-042021-09-272017-12-042021-09-272017-11-06SANTOS, Davi Alves dos. Produção de celulases e xilanases por fermentação em estado sólido usando palha de carnaúba pré-tratada e avaliação da reutilização do resíduo. 2017. 52f. Trabalho de Conclusão de Curso (Graduação em Engenharia Química), Departamento de Engenharia Química, Universidade Federal do Rio Grande do Norte, Natal, 2017.https://repositorio.ufrn.br/handle/123456789/38832Native to Brazil, the Carnaúba palm (Copernicia prunifera) has been used in the production of wax from its leaf. After extraction of the wax, a considerable amount cellulose-rich fibers is generated which can be used as a carbon source for the production of cellulolytic enzymes and bioethanol. However, cellulose is bound to components of complex structures such as hemicellulose and lignin. This causes the production of cellulases from the fermentation by filamentous fungi to be difficult, thus, it is necessary to use a pretreatment to make this process viable. The efficiency of carnauba as carbon source in the in natura form, pretreated with PHA and also recycled (residue reused after passing through a fermentation stage and cellulase production) was evaluated for the production of cellulase using Trichoderma reesei CCT2768 through a solid state fermentation (SSF). Therefore, pretreatment with alkaline hydrogen peroxide (4.35%, pH 11.5) was performed. The in natura, pre-treated and recycled residues were characterized as to their chemical constitution and physical structure (XRD, FTIR and SEM). Then the residues were used for the production of cellulases and xylanases for a fermentation period of 72 hours through SSF. The crude extract produced was used to determine FPase, CMCase, xylanase and total protein activities. Then, mixtures with varying concentrations of some enzymatic extracts obtained were used for the accomplishment of analyzes previously mentioned. The results indicated significant changes in the physical and chemical structure of the residues, mainly in the recycled residue and also in the pre-treated. The crystallinity increases in the pretreated residue to 47.52%, while for the recycled there was a small decrease to 39.87%. The most efficient inducers in cellulase production were PHA-pretreated residues and the mixture of 25% and 75% recycled and pre-treated residues, respectively. Therefore, carnauba shows a promising carbon source capable of inducing the production of cellulolytic and xylanolytic enzymes by T. reesei CCT2768.openAccessperóxido de hidrogênio alcalinoalkaline hydrogen peroxideenzimas celulolíticasbiomassa lignocelulósicapré-tratamentosresíduo recicladocellulolytic enzymeslignocellulosic biomasspre-treatmentsrecycled residuebachelorThesisEngenharias.