Scortecci, Katia CastanhoSilva, Camila Rayane Pereira da2016-12-222022-05-252016-12-222022-05-252016-11-07SILVA, Camila Rayane Pereira da. Análises in silico da sequência SEC14 de cana-de-açúcar para floração. 2016. 46 f. Trabalho de Conclusão de Curso (Graduação)- Centro de Biociências, Universidade Federal do Rio Grande do Norte, Natal, 2016.https://repositorio.ufrn.br/handle/123456789/47298Flowering is considered a key process for plant reproduction and the seed production (the next generation). Although this process is well known in plants, it is not in sugarcane. In previous studies, it was identified a cDNA sequence with homology to SEC14 hence, being a potential repressor for the flowering process in sugarcane. This protein is known for acting in the traffic of phosphatidylcholine and phosphatidylinositol, and it is also associated with the secretory vesicles formation. Then, the aim of the present study was to understand the role of SEC14 sequence in sugarcane flowering. SEC14 protein sequence from sugarcane was used to search for homologous sequences in other plant organisms, as well as for making an interactome. It was also made a search for transcription factors and possible microRNAs (miRNAs) regulatory sites. Furthermore, it was analyzed the expression pattern from SEC14 in plant tissues and other in silico analysis. The results obtained showed the proteins obtained had more than 85% of identity between SEC14 from sugarcane and their homologous. Likewise it was observed in phylogenetic analyses a separation from sequences among the Monocots and Eudicots. The interactomes suggest a probable role of that sequence in the biosynthesis and transport of phosphatidylcholine and phosphatidylinositol. The miRNA data has shown that the SEC14 sequence may have regulation by them, which may be playing a regulatory role in grass and it may be participating in axillary meristem formation as well as in leaf development for A. thaliana model. The analysis of promoter regions showed a possible regulation by phytohormones PHYA and PHYB, development of female gametophyte, cell wall, floral organs and relationship with Abscisic acid (ABA). All these in silico analysis presented here and the Quantitative Real Time Polymerase Chain Reaction (qPCR) results from a previous work reinforce the potential role of SEC14 on sugarcane flowering process, therefore, being a target for genetic improvement.openAccessSECRETION14SECRETION14SaccharumSaccharumFerramentas de bioinformáticaBioinformatics ToolsbachelorThesis