Queiroz, Lelia Maria GuedesVargas, Yailit Del Carmen Martinez2018-05-092018-05-092018-02-15VARGAS, Yailit Del Carmen Martinez. Estudo da expressão imuno-histoquímica das proteínas ING3 e ING4 em lesões odontogênicas epiteliais benignas. 2018. 98f. Dissertação (Mestrado em Patologia Oral) - Centro de Ciências da Saúde, Universidade Federal do Rio Grande do Norte, Natal, 2018.https://repositorio.ufrn.br/jspui/handle/123456789/25156Odontogenic cysts and tumors represent a heterogeneous group of lesions originated from epithelial or ectomesenchymal tissues from the embryonic development of the dental organ, affecting only the gnathic bones. Considering that these epithelial lesions have a distinct biological behavior, they have become the subject of numerous investigations. INGs genes play an important role in cell cycle control, senescence, DNA replication and repair, cell proliferation and apoptosis in various neoplasms. The aim of this study was to comparatively evaluate the immunohistochemical expression of ING3 and ING4 proteins in a series of cases of benign epithelial odontogenic lesions. The sample consisted of 20 odontogenic keratocysts (OK), 20 ameloblastomas (AM) and 15 adenomatoid odontogenic tumors (AOT). The immunohistochemical staining was evaluated semiquantitatively on each case studied according to the percentage of positively immunostained epithelial cells, which ranged from 0 to 3. For statistical analysis were used the Kruskal-Wallis and Spearman tests (p <0.05). Results: Analysis of ING3 expression was positive in the cytoplasm and nucleus of epithelial cells without statistically significant difference between group of lesions studied. Analysis of ING4 expression revealed positivity in the cytoplasm of epithelial cells of all lesions studied. The cytoplasmic immunostaining score for ING4 revealed a statistically significant difference between the groups of lesions studied, with a higher frequency in AOT (p <0.05). After pairing, there was a significant difference between the AM and OK, AM and AOT (p <0.05). And the analysis of ING4 nuclear expression in the studied lesions revealed loss of nuclear expression, followed by reduced nuclear expression for cases of AM and OK, and positivity in the nucleus of AOT epithelial cells. The nuclear immunostaining score for ING4 revealed a statistically significant difference between the groups of lesions studied, with a higher frequency in AOT (p <0.05). After pairing, we observed a significant difference between AM and OK and between AM and AOT and between OK and AOT (p <0.05). In conclusion, the results of the present study suggest that the regulation of ING3 and ING4 in cell growth indicate that these proteins may play an important role in the development and progression of odontogenic lesions with different biological behaviors.Acesso AbertoLesões odontogênicas epiteliais benignasImuno-histoquímicaGene supressor de tumorING3ING4masterThesisCNPQ::CIENCIAS DA SAUDE: PATOLOGIA ORAL