Amaral, Viviane Souza doAlencar, Feliphe Lacerda Souza de2020-07-232020-07-232020-05-04ALENCAR, Feliphe Lacerda Souza de. Bioprospecção da Chromobacterium violaceum para a biorremediação do chumbo: aplicações em biotecnologia e educação em saúde. 2020. 385f. Tese (Doutorado em Desenvolvimento e Meio Ambiente) - Centro de Biociências, Universidade Federal do Rio Grande do Norte, Natal, 2020.https://repositorio.ufrn.br/jspui/handle/123456789/29715The presence of lead in the environment can cause harm not only to ecological balance, but also to human health. Given the above, the need for the development, application and dissemination of methodologies aimed at controlling and reversing this problem is emphasized, such as bioremediation, based on the study of the bioremediation potential of different strains of Chromobacterium violaceum. Theoretical surveys were carried out aimed at a better understanding about the bioremediation of heavy metals in the world scenario, as well as referring to a better knowledge of C. violaceum and its respective biotechnological potentialities. Regarding bioprospecting for lead bioremediation, two strains of the respective bacteria were evaluated, ATCC 12472 provided by the Molecular and Genomic Biology Laboratory of the Federal University of Rio Grande do Norte and the strain called SCV 1, isolated from the Riacho da Cachoeira, located in the municipality of Lajes Pintadas, semi-arid region of Rio Grande do Norte, and impacted, above all, by natural radiation, associated with lead decay. The strains were tested for the pattern of resistance to metal in decreasing concentrations and different exposure times, then evaluating their potential for the in vitro removal of lead by atomic absorption spectrophotometry, as well as their ability to bind to metal, through scanning electron microscopy methodology. The analyzes of proteomics, characterization and comparison of the total protein profile of the respective strains, ATCC and SCV 1 were based on liquid nano chromatography coupled to electrospray ionization and sequential mass spectrometry. The observed data are promising, regarding the feasibility of using C. violaceum as a tool for lead bioremediation. Both strains, ATCC 12472 and SVC 1 showed similar resistance patterns when exposed to the lowest concentrations of lead (0.5 mM to 3 mM). However, the SCV 1 strain was more efficient than the ATCC 12472, for concentrations of 4 to 5 mM. When comparing the efficiency regarding the in vitro removal of the metal in the medium, the SCV 1 was able to remove up to 40% more metal when compared to ATCC 12472. C. violaceum was able to biosorb to lead, without however, undergo changes in its structure. The proteome analysis revealed a total of 1930 proteins in both strains, with several candidate proteins for lead bioremediation being described, related to the biosorption mechanisms; efflux and ionic uptake (bioaccumulation); transport of biomolecules and biomethylation. In addition, were identified bottlenecks proteins, which are essential to the maintenance and homeostasis of the interatoma and played, above all, functions associated with repair, response to stress and acclimatization. Therefore, the changes in the protein profile of C. violaceum, observed in this study, indicate a reprogramming in its metabolism and reveal its important capacity for lead bioremediation.Acesso AbertoChromobacterium violaceumBioprospecçãoBiorremediaçãoMicroscopia eletônicaProteômicaChumbodoctoralThesisCNPQ::CIENCIAS BIOLOGICAS