Luchessi, André DucatiKomatsu, Raquel Costa Silva Dantas2024-02-152024-02-152023-12-15KOMATSU, Raquel Costa Silva Dantas. Expressão de microRNA derivados de leucócitos associada à disfunção sistólica do ventrículo esquerdo em indivíduos que sofreram infarto agudo do miocárdio. Orientador: Dr. André Ducati Luchessi. 2023. 59f. Tese (Doutorado em Ciências da Saúde) - Centro de Ciências da Saúde, Universidade Federal do Rio Grande do Norte, Natal, 2023.https://repositorio.ufrn.br/handle/123456789/57595Left ventricular systolic dysfunction (LVEDD) is considered a serious consequence of ST-segment elevation myocardial infarction (STEMI) and finding biomarkers of LVEDD is necessary. Thus, immune cell-derived microRNAs stand out as they mediate complications associated with AMI. Therefore, this study aims to evaluate the association of leukocyte-derived microRNA with the development of post-STEMI LVSd. Post-STEMI patients were grouped as having (LVSd, n=9) or not LVSd (nonLVSd, n=16). The expression of 61 microRNAs was analyzed by RT-qPCR and the differentially expressed microRNAs were identified. Principal Component Analysis evaluated the stratification of groups based on microRNA expression. Predictive variables of LVSd were investigated through logistic regression analysis. A system biology approach was used to explore the regulatory molecular network of the disease and an enrichment analysis was performed. The let-7b-5p (AUC: 0.807; 95% CI: 0.63– 0.98; p = 0.013), miR-125a-3p (AUC: 0.800; 95% CI: 0.61–0.99; p = 0.036) and miR326 (AUC: 0.783; 95% CI: 0.54–1.00; p = 0.028) were upregulated in LVSd (p < 0.05) and discriminated LVSd from non-LVSd. Multivariate logistic regression analysis showed let-7b-5p (OR: 16.00; 95% CI: 1.54-166.05; p = 0.020) and miR-326 (OR: 28.00; 95% CI: 2.42-323.70; p = 0.008) as predictors of LVSd. The enrichment analysis of the targets of these three microRNAs is associated with immunological response, cell-cell adhesion, and cardiac changes. Therefore, the expression of let-7b-5p, miR326, and miR-125a-3p was associated with post-STEMI LVSd, indicating their potential involvement in the cardiac dysfunction physiopathology and highlighting these microRNAs as possible LVSd biomarkers.Acesso AbertoDisfunção ventricular esquerdaMicroRNAsInfarto do MiocárdioLucócitosInsuficiência cardíacaAnálise de enriquecimentoExpressão de microRNA derivados de leucócitos associada à disfunção sistólica do ventrículo esquerdo em indivíduos que sofreram infarto agudo do miocárdiodoctoralThesisCNPQ::CIENCIAS DA SAUDE