Rocha, Hugo Alexandre de OliveiraBerto, Isabelle Luna de Oliveira Dantas2024-07-042024-07-042024-04-26BERTO, Isabelle Luna de Oliveira Dantas. Aprimorando o potencial antioxidante e protetor da Xilana de sabugo de milho por meio da conjugação com ácido gálico: uma abordagem inovadora. Orientador: Dr. Hugo Alexandre de Oliveira Rocha. 2024. 46f. Dissertação (Mestrado em Ciências da Saúde) - Centro de Ciências da Saúde, Universidade Federal do Rio Grande do Norte, Natal, 2024.https://repositorio.ufrn.br/handle/123456789/58586Maize ranks as the second most widely produced crop globally, yielding approximately 1.2 billion tons, with corn cob being its primary byproduct, constituting 18 kg per 100 kg of corn. Agricultural corn production generates bioactive polysaccharide-rich byproducts, including xylan (Xyl), that already demonstrated antioxidant activity in previous studies. In this study, we used the redox method to modify corn cob xylan with gallic acid, aiming to enhance its antioxidant and protective capacity against oxidative stress. The conjugation process resulted in a new molecule termed conjugated xylan–gallic acid (Xyl-GA), exhibiting notable improvements in various antioxidant parameters, including total antioxidant capacity (1.4-fold increase), reducing power (1.2-fold increase), hydroxyl radical scavenging (1.6-fold increase), and cupric chelation (27.5-fold increase) when compared with unmodified Xyl. At a concentration of 1 mg/mL, Xyl-GA demonstrated no cytotoxicity, significantly increased fibroblast cell viability (approximately 80%), and effectively mitigated intracellular ROS levels (reduced by 100%) following oxidative damage induced by H2O2. Furthermore, Xyl-GA exhibited non-toxicity toward zebrafish embryos, offered protection against H2O2-induced stress, and reduced the rate of cells undergoing apoptosis resulting from H2O2 exposure. In conclusion, our findings suggest that Xyl-GA possesses potential therapeutic value in addressing oxidative stress-related disturbances. Further investigations are warranted to elucidate the molecular structure of this novel compound and establish correlations with its pharmacological activities.Acesso AbertoZea mays (milho)Xilana de sabugo de milhoAntioxidanteZebrafishAprimorando o potencial antioxidante e protetor da Xilana de sabugo de milho por meio da conjugação com ácido gálico: uma abordagem inovadoramasterThesisCNPQ::CIENCIAS DA SAUDE