Farias, Kleber Juvenal SilvaAlmeida Júnior, Renato Ferreira de2016-04-282016-04-282015-03-16ALMEIDA JÚNIOR, Renato Ferreira de. Avaliação do potencial antiviral do extrato bruto da planta Caesalpinia echinata e da rifampicina contra vírus dengue-2 em cultura de células. 2015. 67f. Dissertação (Mestrado em Ciências Biológicas) - Centro de Biociências, Universidade Federal do Rio Grande do Norte, Natal, 2015.https://repositorio.ufrn.br/jspui/handle/123456789/20321The dengue virus belongs to the Flaviviridae family and the Flavivirus genus, consisting of four serotypes antigenically distinct, are considered the most important arbovirus in the world to cause high rates of morbidity and mortality in tropical and subtropical regions of the world, threatening to 3, 6 billion people in over 100 countries. Because it is a disease with a wide clinical spectrum and has no vaccine or effective treatment, the study of possible antiviral drugs aimed at reducing viremia of patients is of paramount importance, since this is one of the factors which can lead to dengue hemorrhagic fever and dengue shock syndrome that are severe forms of the disease. In the present study we evaluated the antiviral potential of the leaf extract of the plant Caesalpinia echinata against dengue-2 virus (DENV-2) in cultured C6/36 and Vero cells and the antiviral action of Rifampicin on Vero cells. The choice of Caesalpinia echinata is due to the fact that has been observed its action anti-inflammatory and antimalarial , and not have been found no study evaluated its antiviral effect. Rifampin was chosen was chosen for demonstrated antiviral action, especially against poxviruses, but few sane reports usage of this drug against RNA viruses. The result was obtained by quantifying the viral load bythe technique of Real-time qRT-PCR. Cells infected with DENV-2 were subjected to treatment for 7 days in different concentrations of plant extract Caesalpinia echinata (0.68 - 0,0068mg / ml). As a result, we could not observe a inhibition significant of virus replication in both cell cultures. The Rifampicin was used for different treatment condition,which were evaluated over 72 hours the amount of viral load produced in Vero cells, In the conditions treatment and the test virucidal theantiviral activity, which is capable of reducing the rate of 100X replication as compared to control. According to our results we can conclude that Rifampicin was effective in action against to infection DENV-2 in Vero cell culture.porAcesso AbertoDENV-2células C6/36 e VeroCaesalpinia echinataRifampicinaQuantificaçãoqRT-PCR em tempo real e PFUmasterThesisCNPQ::CIENCIAS BIOLOGICAS