Santos, Everaldo Silvino dosCavalcanti, Jorge dos SantosMagalhães, Margarida Maria dos Anjos2021-06-142021-06-142009CAVALCANTI, J. S. MAGALHAES, M. M. A.; SANTOS, E. S.. Recuperação de proteína do soro de leite bovino usando cromatografia de interação hidrofóbica em leito expandido. Higiene Alimentar, v. 23, p. 114-118, 2009. Disponível em: http://higienealimentar.com.br/178-2/. Acesso em: 20 abr. 2021.0101-9171https://repositorio.ufrn.br/handle/123456789/32671The present work deals with of cheese serum type “curdle” protein adsorption on the resin of hydrophobic interaction StreamlineTM Phenyl specially manufactured for expanded bed applications. A column of 2.6 cm of diameter for 30 cm of height, connected to a peristaltic pump was used. A sample of serum solution (250 mL) was applied to a column containing the resin StreamlineTM Phenyl (25 mL) previously equilibrated with in Tris/HCl (50 mM, pH 7.0) with NaCl (1.0 M). After washing step (300 ml of Tris/HCl 50 mM, pH 7.0 with NaCl (1.0M)) elution was carried out in buffer Tris/HCl (50 mM, pH 7.0) at room temperature with a fixed bed height of 5.0 cm. The results showed that it was possible to recover 37% of proteins initially contained in the serum just using one stageAttribution-NonCommercial-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/Interação hidrofóbicaSoroProteínasHidrophobic interactionProteinsWheyarticle