Silva, Marcelo SousaAraujo, Isadora Bezerra Menezes de2020-12-192021-09-202020-12-192021-09-202020-12-04ARAUJO, Isadora Bezerra Menezes de. Caracterização da imunogenicidade de proteínas recombinantes quiméricas de Trypanosoma cruzi em modelo murino. 2020.27f. Trabalho de conclusão de curso (Graduação em Farmácia) - Departamento de Análises Clínicas e Toxicológicas, Universidade Federal do Rio Grande do Norte, Natal, 2020.https://repositorio.ufrn.br/handle/123456789/35794Chagas disease (CD) is a pathology of worldwide concern caused by the pathogen Trypanosoma cruzi. Thus, it is necessary to adopt more effective measures in order to reduce the spread of this disease, that is why an immunization protocol was developed with a mix of four chimeric recombinant proteins called IBMP-8.1; IBMP-8.2; IBMP-8.3; IBMP-8.4 associated or not with the aluminum hydroxide adjuvant, aiming to characterize yours immunogenic capacity for protection against T. cruzi. The female BALB / c mice were immunized and blood samples were obtained at 15, 30 and 45 days after immunization. The profile of total IgG antibodies and their subclasses, IgG1 and IgG3, were determined by enzymatic immunoassay. The data report the highest production of total anti-IBMP-8.1 IgG; 8.2; 8.3; 8.4 in the group in association with aluminum hydroxide (MixIBMP + Alum), whereas the MixIBMP group alone did not show significance in relation to the negative control. However, in the IgG1 subclass there was production of antibodies in both groups, being higher in MixIBMP + Alum and lower in the group without aluminum, which stands out when analyzed in relation to the IgG3 subclass. In conclusion, the presence of immunogenicity in this immunization protocol related to these proteins is clear.Doença de ChagasProteínasMixIBMPHidróxido de alumínioAnticorposImunogenicidadebachelorThesis