Araújo, Renata MendonçaSantos, Sarah Pollyana Dias dos2025-07-212025-07-212024-10-30SANTOS, Sarah Pollyana Dias dos. Aspidosperma nitidum Benth e Aspidosperma pyrifolium Mart. (Apocynaceae): estudo fitoquímico, avaliação de atividade biológica e revisão dos dados de RMN de alcaloides β-carbolina. Orientadora: Dra. Renata Mendonça Araújo. 2024. 362f. Tese (Doutorado em Química) - Centro de Ciências Exatas e da Terra, Universidade Federal do Rio Grande do Norte, Natal, 2024.https://repositorio.ufrn.br/handle/123456789/64754The genus Aspidosperma (Apocynaceae) is notable for the presence of indole alkaloids, characterized by structural diversity and interesting biological activities. The species Aspidosperma nitidum Benth and Aspidosperma pyrifolium Mart., popularly known as carapanaúba and pereiro, respectively, are native to the Amazon region and the Caatinga biome. These species have applications in traditional medicine, primarily due to their antiinflammatory, antimalarial, and antimicrobial properties. The present study aimed to investigate the phytochemical properties of A. nitidum and A. pyrifolium, as well as the safety of their extracts and the anti-inflammatory and healing properties of the hydroethanolic extract of Aspidosperma nitidum Benth stem bark (ANCCE). Additionally, a literature review on indole alkaloids of the β-carboline type was conducted. The study is divided into chapters as follows: Chapter I: Provides a brief introduction, theoretical background on the studied species, and the objectives of the research. Chapter II: Presents a literature review on β-carboline-type alkaloids isolated from natural sources, with or without reported biological activities, between 1992 and 2023. The data were collected from Scifinder and ScienceDirect databases. A total of 300 indole alkaloids of the β-carboline type were cataloged with 1D NMR data, classified into 4 main classes and 13 subclasses according to their structural diversity. Of these, 82 bioactive compounds were identified, distributed across 92 species and 50 families, aiding in dereplication studies of species and these alkaloids. Chapter III: Describes the dereplication study of A. nitidum and A. pyrifolium species using UPLC-MS/MS and GC/MS techniques. However, only the species A. pyrifolium was analyzed using both methods. Nevertheless, fragmentation patterns of the annotated indole alkaloids were proposed for both techniques. This study enabled the identification of 21 indole alkaloids and the verification of fragmentation patterns in most of them. Chapter IV: Reports the phytochemical study and evaluation of the anti-inflammatory and healing activity of the hydroethanolic extract of Aspidosperma nitidum Benth bark. Conducted in collaboration with the Laboratory of Pharmaceutical Technology and Biotechnology (TECBIOFAR) and the Federal University of Ceará (UFC), this project led to the isolation of an isocoumarin and sucrose glycoside, both known in the literature but isolated for the first time in A. nitidum. A new glycosylated 1-benzazepine derivative was also proposed and identified for the first time in the literature. Biological activity assays, including MTT and hemolysis tests, demonstrated no cytotoxicity at any evaluated extract concentration. The acute toxicity model showed no signs of toxicity or significant behavioral, biochemical, or hematological changes after extract use. In the edematogenic model, A. nitidum extract significantly reduced edema percentage as well as MPO levels and pro-inflammatory cytokines. It was also effective in reducing leukocyte migration, total proteins, MPO, and cytokine concentrations in the zymosan-induced air pouch model. Furthermore, the extract exhibited healing properties, reducing the area of skin wounds.pt-BRAcesso AbertoQuímicaAspidosperma nitidumAspidosperma pyrifoliumAlcaloides indólicosβ-carbolinasAnti-inflamatóriodoctoralThesisCIENCIAS EXATAS E DA TERRA::QUIMICA