Oliveira, Riva de PaulaSilva, Giovanna Melo Martins2018-12-172021-10-062018-12-172021-10-062018-11-26SILVA, Giovanna Melo Martins. Caracterização dos mecanismos subjacentes da ação neuroprotetora do extrato de guaraná (Paullinia cupana) no organismo modelo Caenorhabditis elegans. 2018. 74 f. Monografia (Graduação em Biomedicina) – Curso de Biomedicina, Centro de Biociências, Universidade Federal do Rio Grande do Norte, Natal, 2018.https://repositorio.ufrn.br/handle/123456789/43185Investigations involving the guarana seeds extract (Paullinia cupana) show many benefic proprieties, including antioxidative, antimutagenic and protective and stimulating on the central nervous system (CNS). Preliminary results have shown that guarana extract delays the paralysis profile induced by β-amyloid peptide overexpression in Caenorhabditis elegans, besides being able to reduce the neurotoxicity of Huntingtin protein (Q150), to have an antioxidant effect by reducing ROS in animals expressing the βA peptide and even increasing proteasome activity. In the present study, the nematode C. elegans was used as a model organism to better understand the mechanisms involved in the neuroprotective action of guarana hydro-alcoholic extract (GHE). The treatment with GHE didn’t increase the animal’s survival rates under oxidative stress conditions induced by tert-butyl hydroperoxide (t-BOOH), however, was able to increase the medium and maximum lifetime, the pharyngeal pumping rates and motility under normal conditions, without interfering with reproduction. By counting fluorescence focal points in the LGG-1::GFP transgenic lineage, GHE treatment demonstrated ability to increase lysosomal activity. The GHE mechanism of action dependence of transcriptional factors HSF-1, SKN-1 and DAF-16 was analyzed through transgenic lineage CL2006 unc-54/βA1-42 human peptide (Alzheimer’s disease model) and interference RNAi (RNAi) technology in bacterial vehicle with the transgenic E. coli HT115 (PL440). The protective effect of guarana showed partial dependence of transcriptional factors SKN-1 and DAF-16, but not of HSF-1. In conclusion, guarana has the potential to activate stress response pathways and processes involved in protein homeostasis, presenting itself as an option for treatment and prevention of neurodegenerative diseases.Attribution-NonCommercial-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/GuaranáPaullinia cupanaCaenorhabditis elegansEstresse oxidativoMal de AlzheimerLongevidadeGuaranaOxidative stressAlzheimer's diseaseLongevitybachelorThesis2.02.05.00-7 Genética Humana e Médica 2.02.06.00-3 Mutagênese