Silbiger, Vivian NogueiraLavieri, Giovani Jardini2025-11-032025-11-032025-06-10LAVIERI, Giovani Jardini. O papel dos miRNAs na doença de Lyme: uma revisão. Orientadora: Dra. Vivian Nogueira Silbiger. 2025. 58f. Dissertação (Mestrado em Ciências Farmacêuticas) - Centro de Ciências da Saúde, Universidade Federal do Rio Grande do Norte, Natal, 2025.https://repositorio.ufrn.br/handle/123456789/66002Introduction: Lyme disease (LD) is the most common tick-borne disease in North America and Europe. Caused by the bacterium Borrelia burgdorferi, it manifests itself in different forms and stages, and may be asymptomatic or with nonspecific symptoms, in addition to the difficulty in localizing the arachnid bite and the possible lack of formation of erythema migrans, which makes clinical diagnosis difficult. Current laboratory diagnostic methods have limitations, such as the absence of antibodies against bacteria during the first weeks of infection, in addition to access to samples, since Borrelia burgdorferi lodges in difficult-toaccess tissues. In this context, miRNAs emerge as potential candidates in understanding the pathophysiology of LD, as well as biomarkers and therapeutic targets, given their stability and ability to reflect pathological states, including bacterial infections. Objective: To conduct a narrative review on the role of miRNAs in LD. Methodology: The search was performed in PubMed, Embase, ScienceDirect, Web of Science and LILACS databases, using the terms "Lyme Disease", "Borrelia burgdorferi", "Lyme Borreliosis", "Lyme Arthritis", "Lyme Carditis" and "MicroRNA". Results: Of the 7 studies included, three were performed in vitro with different human cell cultures, two used in vivo models with mice, one study was performed in vitro with Ixodes scapularis ticks and one was observational with patient samples. Total RNA extraction methodologies varied between the TriZol method and the miRNeasy Kit and the analysis of miRNA expression varied from RNAseq to qRT-PCR. In total, 261 differently expressed miRNAs were analyzed among all studies and, of these, miR-146a-5p, miR-155, miR-145 and miR-146b were described in more than one study with increased expression in different samples and clinical manifestations. Conclusion: miRNAs such as miR-146a-5p, miR-155, miR-145, and miR-146b play important roles in the inflammatory regulation of LD, being involved in dermal manifestation, arthritis, carditis, and microglial activation. These miRNAs have potential as biomarkers and therapeutic targets in LD, although further studies are needed to validate their applicability in the diagnosis and treatment of the disease.pt-BRAcesso AbertoDoença de LymeMicroRNAsBorrelia burgdorferiArtrite de LymeCardite de LymemasterThesisCIENCIAS DA SAUDE::FARMACIA