Guedes, Paulo Marcos da MattaQueiroga, Tamyres Bernadete Dantas2022-12-122022-12-122022-10-19QUEIROGA, Tamyres Bernadete Dantas. Avaliação de novas abordagens para controle e diagnóstico da leishmaniose visceral no Brasil. Orientador: Paulo Marcos da Matta Guedes. 2022. 141f. Tese (Doutorado em Ciências Farmacêuticas) - Centro de Ciências da Saúde, Universidade Federal do Rio Grande do Norte, Natal, 2022.https://repositorio.ufrn.br/handle/123456789/50100Visceral leishmaniasis is a serious and chronic disease with a geographic spread in Brazil due to the absence of a treatment that eliminates the parasite, ineffective control measures, and diagnostic methods with low accuracy. The study aimed to evaluate the systemic insecticidal activity of a single oral dose of fluralaner against Lutzomyia longipalpis, as well as evaluate the mid-infrared as an alternative for Leishmania infantum detection in canine serum samples. Mongrel dogs were recruited and randomized into two groups, fluralaner (n = 4) and control (n = 4), and female specimens of Lu. longipalpis were allowed to feed on all dogs before treatment, on day one post-treatment, and then monthly until 1-year post-treatment. In addition, attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR) combined with multivariate analysis was employed to distinguish samples from dogs infected with L. infantum in different clinical forms (n = 29) from healthy animals (n = 30). After data analysis, there was 100% mortality of Lu. longipalpis for up to five months after treatment initiation. The efficacy of fluralaner ranged from 100% on day 1 to 68% at six months with a decrease to 1.4% at one-year post-treatment. ATR-FTIR was efficient in differentiating samples from infected and uninfected dogs with 100% sensitivity and specificity using the successive projections algorithm with linear discriminant analysis (SPA-LDA), SPA with support vector machine (SPA-SVM), and the genetic algorithm with LDA (GA-LDA). These satisfactory results suggest that fluralaner may be used as a control strategy for VL in endemic areas, as well as spectroscopy associated with multivariate analysis demonstrates its potential as an alternative tool for the VL diagnosis in dogs.Acesso AbertoLeishmaniose visceralFluralanerLutzomyia longipalpisLeishmania infantumEspectroscopiaQuimiometriadoctoralThesisCNPQ::CIENCIAS DA SAUDE::FARMACIA