Fernandes, José VeríssimoAbrantes, Jayra Juliana Paiva Alves2017-02-162017-02-162016-03-22ABRANTES, Jayra Juliana Paiva Alves. Avaliação do perfil da resposta imune presente na mucosa da cérvice uterina de mulheres infectadas pelo HPV. 2016. 82f. Dissertação (Mestrado em Ciências Biológicas) - Centro de Biociências, Universidade Federal do Rio Grande do Norte, Natal, 2016.https://repositorio.ufrn.br/jspui/handle/123456789/22006Genital human papillomavirus (HPV) infection is one of the most common causes of sexually transmitted diseases in both genders, with a higher infection prevalence among young women sexually active, consisting in a serious public health problem. The persistent high-risk HPV infection with epigenetic events triggered by exposure to physical, chemical, environmental, beyond the dysfunction of the immune system, can favor the development of premalignant and malignant lesions of the uterine cervix. The objective of this study was estimate the HPV prevalence and evaluate the profile of the immune response in women, with and without cervical lesions associated with it; through the expression levels of cytokines, TLR9 and Myd88 in the uterine mucosa, establishing a correlation with the presence or absence of lesions and HPV. The study included women aged between 16 and 85 years, with an average of 36,2 years and standard deviation of 11.26. A total of 225 women participated of this study, 126 were recruited among women attending the screening program for cervical cancer and the other 99 were sent to maternity Januário Cicco with a suspected uterine cervix injury. Patients who agreed to participate, were submitted to an interview to obtain socio-demographic data, followed by clinical examination and collection of two cervical scrape specimens, one for cytological analysis and another for molecular analysis in order to detect HPV. Colposcopy was performed in women who had suspected injury to detect these possible changes. It was collected two fragments of tissue in each biopsy, one for histopathological analysis and other to obtain nucleic acid, destined to detection of HPV by conventional PCR and, analysis of receptors and cytokines expression involved in the immune response by real-time PCR. The overall HPV prevalence was 39.5%, 61,1% in patients with lesions and 18,8% % in patients without lesions. Women with multiple partners, and those who had two or more pregnancies had higher risk of HPV infection. There was a correlation between cervical changes and HPV infection. The RNA-m expression levels of The pro-inflammatory cytokines INF-γ, TNF-α, IL-17 and the anti-inflammatory IL-10 showed higher expression levels in the positive HPV injury group compared to uninjured, compared to control. It is possible that the increased expression of cytokines, especially IL-10 and IL-17 is associated with a chronic inflammatory process present in the lesions and can be related to favor viral persistence and likely to progress to malignant lesions.Acesso AbertoHPVCitocinasImunidadeDoença do colo do úteromasterThesisCNPQ::CIENCIAS BIOLOGICAS