Abreu, Luiz Roberto Diz deGomes Júnior, José Edilson2014-12-172007-02-132014-12-172006-12-06GOMES JÚNIOR, José Edilson. Purificação e caracterização de uma ß-N-acetillhexosaminadase extraída do mamífero marinho Sotalia fluviatilis. 2006. 80 f. Dissertação (Mestrado em Bioquímica; Biologia Molecular) - Universidade Federal do Rio Grande do Norte, Natal, 2006.https://repositorio.ufrn.br/jspui/handle/123456789/12612This report shows 2232 times purification of a βNAcetylhexosaminidase from hepatic extracts from the sea mammal Sotalia fluviatilis homogenate with final recovery of 8,4%. Sequenced steps were utilized for enzyme purification: ammonium sulfate fractionation, Biogel A 1.5 m, chitin, DEAESepharose and hydroxyapatite chromatographies. The protein molecular mass was estimated in 10 kDa using SDSPAGE and confirmed by MALDITOF. It was found to have an optimal pH of 5.0 and a temperature of 60°C. Using pnitrophenylNAcetylβDglycosaminide apparent Km and Vmax values were of 2.72 mM and 0.572 nmol/mg/min, respectively. The enzyme was inhibited by mercury chloride (HgCl2) and sodium dodecil sulfate (SDS)application/pdfAcesso AbertoβNAcetilhexosaminidaseβNAcetilglucosaminidaseβNAcetilgalactosaminidaseSotalia fluviatilisGlicosaminoglicanosExtratos hepáticosMamíferos marinhosβNAcetylhexosaminidaseβNAcetylgalactosaminidaseβNAcetylglycosaminidaseSotalia fluviatilisGlycosaminoglycansHepatics extractsSea mammalmasterThesisCNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA