Langassner, Silvana Maria ZucolottoFernandes, Julia Morais2020-01-232020-01-232019-07-26FERNANDES, Julia Morais. Bryophyllum pinnatum e Kalanchoe laciniata: estudo fitoquímico, metabolômico e avaliação da inibição da atividade fosfolipásica da peçonha de Bothrops erythromelas de extratos obtidos sob diferentes condições de cultivo. 2019. 249f. Tese (Doutorado em Ciências Farmacêuticas) - Centro de Ciências da Saúde, Universidade Federal do Rio Grande do Norte, Natal, 2019.https://repositorio.ufrn.br/jspui/handle/123456789/28365Kalanchoe laciniata (L.) DC. and Bryophillum pinnatum (Lam) Pers. (Crassulaceae) known populary as saião or coirama are used in Brazil, mainly in the Northeast region, and their use remarks in the anti-inflammatory problems treatment. Regarding chemical composition, patuletin, kaempferol and quercetin glycosylates derivates are the main compounds decribed, however the active compounds are not known. Besides, there are not enough control quality studies that can be contribute to authentication and differentiation between K. laciniata and B. pinnatum, especially by High or Ultra Performance Liquid Chromatography (HPLC or UPLC) coupled Mass Spectrometer (MS) and/or Nuclear Magnetic Resonance (NMR). Once B. pinnatum is in Renisus (Relação Nacional de Plantas Medicinais de Interesse do SUS), it is necessary to increase studies with the both species to ensure safety, efficacy and quality of the possible herbal drug can be developed and distributed by SUS. In this context, the proposal is performed a metabolomic and pharmacologic approach with K. laciniata and B. pinnatum species. To achieve this goal, the methodology was divided: i: isolation and characterization of main compounds of leaves extracts of both species; ii: quantification of chemical markers; iii: metabolomic approach of the extracts in response to stress induced by drought and salt stress; iv: in vitro assays to evaluate the inhibitory effects of the extracts against the enzymes that trigger the Bothrops erythromelas envenomation. The most significant results were i. by the centrifugal partition chromatography (CPC) technique application, it was possible to isolate quickly and efficiently the majors flavonoids of B. pinnatum, besides it has enabled the isolation of substance not described for the genus, named sarmentosin; ii. a) to quantify three flavonoids in the extract of B. pinnatum by UPLC-DAD and to suggest the flavonoid quercetin 3-O-α-L-arabinopyranosyl-(1→2)-O-α-Lrhamnopyranoside as a specific marker it, and b) to develop and validate an NMR methodology to quantify sarmentosin in the extract of B. pinnatum; iii. metabolomic approach obtained by UPLC-MS/MS data showed that drought and salinity conditions can influence production of different metabolites, indicating that both species are resistant to watering suspension up to the 20th day, but that the lower amount of salinity in the irrigation water already induces differences in metabolic production, mainly phenolic compounds; iv. in vitro activities showed the potential of the species in inhibiting PLA2, an important toxin in poisoning, and showed that extracts obtained from saline and water stress directly influence its inhibition. In this context, this work represented a chemical and pharmacological advance in the study of these species. Thus, through an efficient and green methodology developed by CPC, the markers of B. pinnatum extract in large quantities were obtained, as well as the validation of a method to quantify them according to the parameters of RDC 166/2017, until so not found in the literature. In addition, it was possible to suggest certain cultivation conditions that influence the production of metabolites by plants, possibly linked with the antivenom activity presented by extracts of both species.Acesso AbertoCrassulaceaeMetabolômicaMarcadores químicosFitoterápicodoctoralThesisCNPQ::CIENCIAS DA SAUDE::FARMACIA