Lanza, Daniel Carlos FerreiraMendes, Cayro de Macêdo2019-03-122019-03-122018-11-19MENDES, Cayro de Macêdo. Caracterização in silico de orfs variáveis e de regiões regulatórias no genoma do vírus da síndrome da mancha branca (WSSV). 2018. 79f. Dissertação (Mestrado em Bioinformática) - Instituto Metrópole Digital, Universidade Federal do Rio Grande do Norte, Natal, 2018.https://repositorio.ufrn.br/jspui/handle/123456789/26750The white spot syndrome virus (WSSV) is one of the biggest problems facing global shrimp farming, causing considerable economic losses. The WSSV genome presents some coding regions that vary between the different isolates. Those regions named wsv129 (ORF75), wsv178 (ORF94), wsv249 (ORF125), wsv461 / 464 (ORFs 14/15) and wsv477 / 502 (ORFs 23/24) are possibly involved in virulence mechanisms but have not been fully characterized functionally so far. The in silico characterization has been used as a more accessible alternative for prediction and study of the protein structure that does not have the crystallographic structure available. This work aimed to characterize in silico the putative proteins encoded by the variable regions of the WSSV genome, in order to identify possible functions. The variable regions of the ORs wsv129, wsv178, wsv249 and the clusters formed by the ORs wsv461/464 and wsv477/502 were analyzed phylogenetically, and structurally. The amino acid sequences were subjected to remote homologous searches of motifs, conserved domains, fold recognition and prediction of secondary and tertiary structures. It was possible to model tertiary structures of protein domains and infer possible functions for the ORs wsv463a (formin like - regulation of actin filaments), wsv477 (interaction with RNA - posttranscriptional processes), wsv479 and wsv497 (XPD like - helicase), wsv 492 (hemagglutinin like signaling, viral docking) and wsv249 (ankyrim repeat and RING-H2 domains - modulation of Ubiquitin-dependent proteolysis), besides proposing structural functions for the ORs wsv129 and wsv178 due to their structural characteristics and charge. It was also possible to detect signatures associated with nuclear localization signals within the repeating units of the sequences encoded by the ORs wsv129 and wsv178. In addition to protein structure analyzes, some 100 and 200 nt upstream regulatory regions were prospected for the coding regions and it was possible to detect some motifs, including a Zinc-Finger binding site, suggesting the interaction between possible transcription factors. From the results it was proposed a model of performance for each of the proteins studied.Acesso AbertoPenaeus vannameiCamarãoWhispovirusORF94ORF75ORF125VNTRsmasterThesisCNPQ::CIENCIAS BIOLOGICAS: BIOINFORMÁTICA