Andrade, Vânia SousaCosta, Sara Ester de Lima2019-05-092019-05-092019-03-08COSTA, Sara Ester de Lima. Atividade antiproliferativa e citotóxica do óleo essencial e extrato hidroalcóolico provenientes da Lippia grata SCHAUER. 2019. 73f. Dissertação (Mestrado em Biologia Parasitária) - Centro de Biociências, Universidade Federal do Rio Grande do Norte, Natal, 2019.https://repositorio.ufrn.br/jspui/handle/123456789/27046The Caatinga is a rich biome with a flora well adapted to its predominant climate, the semi-arid, where some of these plants have medicinal properties, among them the Lippia grata (formerly Lippia gracilis), known as alecrim-da-chapada. This specie of the Verbenaceae family is usually used to treat throat and stomach conditions through teas and infusions. The objective of the present study was to evaluate the cytotoxicity and anti-proliferative activity resulting from the use of the essential oil and hydroalcoholic extract of L. grata in cell line of gastric adenocarcinoma (AGS) in cell. For this, cellular viability was evaluated by the reduction of (3-(4,5-Dimethylthiazol-2- yl)-2,5-Diphenyltetrazolium Bromide (MTT), clonogenic assay and flow cytometry, as well as the analysis of the chemicals components present in the essential oil through gas chromatography associated with mass spectrometry. Cell viability was assessed at 24 h, 48 h and 72 h at the concentrations of 125, 250, 500, 750 and 1000 μg/mL for MTT reduction in both AGS cells and normal murine fibroblast cell line (3T3). Similar cell death for AGS was observed in 48 h and 72 h and discreetly in 24 h, both in relation to oil and extract; the 3T3 line presented cell death only at the highest concentration in the initial period, and for 48 h and 72 h similar values from the concentration of 500 μg/mL. The clonogenic assay reveals a 100% reduction in tumor cell proliferation (AGS). Flow cytometry revealed that the treatment of AGS cells at 500 μg/mL for 48 h was better using the oil over the extract, revealing 54.4% and 14% cells in late apoptosis, respectively. The major components of the phytochemical analysis of the essential oil were carvacrol (49.9%) followed by thymol (15.7%). The results reveal a high anti-proliferative and anti-carcinogenic potential of the essential oil and L. grata extract against the AGS line derived from gastric cancer, by the induction of apoptosis when compared to 3T3 cells, suggesting its selective cytotoxicity for malignant cells. These results are promising and motivate the realization of new studies with the products obtained from L. grata leaves, in order to evaluate pharmacologically if feasible in the treatment of gastric disorders.Acesso AbertoAdenocarcinoma gástricoCarvacrolTimolAlecrim-da-chapadamasterThesisCNPQ::CIENCIAS BIOLOGICAS: BIOLOGIA PARASITÁRIA