Lanza, Daniel Carlos FerreiraOliveira, Maryana Thalyta Ferreira Câmara2022-04-052022-04-052022-02-11OLIVEIRA, Maryana Thalyta Ferreira Câmara de. Identificação de patógenos com genoma de DNA em um metagenoma de sêmen humano. 2022. 81 f. Monografia (Graduação em Biomedicina) – Universidade Federal do Rio Grande do Norte, Natal, 2022.https://repositorio.ufrn.br/handle/123456789/46797Infertility is a growing factor worldwide and has generated a great demand for assisted reproduction. Among the main etiological factors responsible for this infertility are infectious and inflammatory conditions. A total of 50% of all infertility described, in combination or alone, is due to the infertility of the male factor. In this study, the metagenomic approach and PCR were used to identify potential pathogens, with DNA genome, in seminal samples from patients undergoing assisted reproduction treatment. For the analysis, the DNA of a semen pool was evaluated by shotgun sequencing and the fragments were processed and aligned using BLASTn. In taxonomic characterization, approximately 25 genera and 120 species of bacteria were detected in the seminal sample and Gardnerella vaginalis was the bacterial species that presented the highest number of hits. Also in this study, 4 species of virus were identified, including HERVs, HHV-5 and HPV-16 and 2 species of protozoa, namely Plasmodium vivax and Toxoplasma gondii. In molecular detection, HPV and Toxoplasma gondii were negative, however, a pattern of positive bands was detected for the latter with the 18S primers. Finally, it was found that, as the research of the seminal microbiome is an approach of increasing scientific interest, the results obtained can be explored in future studies so that molecular protocols can be developed that facilitate the identification of these pathogens.Attribution-NonCommercial-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/Microbioma humanoAnálise seminalMetagenômicaInfertilidade masculinaDiagnósticoReprodução assistidaHuman microbiomeSeminal analysisMetagenomicMale infertilityDiagnosisAssisted reproductionbachelorThesis