Medeiros, Caroline Addison Carvalho Xavier deTavares, Emanuella de Aragão2019-03-082019-03-082018-10-15TAVARES, Emanuella de Aragão. Efeito da membrana quitosana com complexo de Zinco-vanilina no tratamento de lesões cutâneas em ratos diabéticos. 2018. 59f. Dissertação (Mestrado em Ciências Biológicas) - Centro de Biociências, Universidade Federal do Rio Grande do Norte, Natal, 2018.https://repositorio.ufrn.br/jspui/handle/123456789/26701The treatment of chronic wounds is still considered a public health problem, when it affects groups of risks as diabetics and becomes a great clinical challenge. In this work, we evaluated the healing effects of a new zinc complex associated with a chitosan membrane, tested in cutaneous wounds of diabetic rats. Wistar rats were submitted to induction of diabetes with streptozotocin 40 mg / kg (i.v.). On the seventh day after diabetic induction, a circular skin excision (1.0 cm) with punch was performed. The lesions were treated with the pure chitosan membrane and with the membrane associated with the Zinc-vanillin complex (CS-ZPV1 and CS-ZPV2) at two concentrations, 7.36 x 10-7 mol cm-2 and 1.22 x 10-6 mol cm -2, respectively. The samples were submitted to macroscopic, histopathological, cytokine (TNFα, IL-1β and IL-10) and reverse transcriptase polymerase chain reaction (RtPCR) (TGFβ and VEGF) assays. The analysis revealed reepithelialization, angiogenic stimulation, collagen deposition and expression of proteins that aid in the healing process in a significant proportion in the groups treated with the CS-ZPV membrane compared to the groups without treatment. Treatment with CS-ZPV reduced the levels of TNF-α, IL-1β and increased IL-10, also increased the gene expression of TGFβ and VEGF. The evaluated parameters suggest that CSZPV in the two concentrations tested may be effective in the treatment of chronic wounds.Acesso AbertoDiabetesLesões cutâneasCicatrização de feridasZincoMembrana de quitosanamasterThesisCNPQ::CIENCIAS BIOLOGICAS