Amaral, Viviane Souza doOliveira, Rebeka Guerra de2019-06-262021-10-062019-06-262021-10-062019-05-28OLIVEIRA, Rebeka Guerra de. Efeitos da radiação ionizante natural na metilação global do genoma da população de Lajes Pintadas/RN. 2019. 105f. Trabalho de Conclusão de Curso (Graduação em Biomedicina) - Centro de Biociências, Departamento de Biologia Celular e Genética, Universidade Federal do Rio Grande Norte, Natal, 2019.https://repositorio.ufrn.br/handle/123456789/43192Ionizing radiation is ubiquitous and is part of the daily lives of people, but in high doses brings several damages to people's health, an example of this fact can be observed in the municipality of Lajes Pintadas (RN) which has the problem of a great concentration of radon gas in the air, this is radioactive and is derived from the uranium decay, a fact noted in the region is the high incidence of cancer mortality. It is possible to relate damage caused by IR with changes in epigenetics, for it was analyzed the methylation of LINE-1, the longest repeating element of DNA, which because of your high number of repetitions provides an idea of the panorama of global methylation of the genome. The purpose of this study was to evaluate the epigenetic changes caused by natural high ionizing radiation in residents of Lajes Pintadas/RN. 104 individuals were recruited, between the control and experimental groups. After the consent and clarification of the research, questionnaires were applied and the participants' DNA was extracted to analyze the effect of IR on the methylation of the LINE -1 gene through the COBRA protocol. with bisulphite conversion, PCR, restriction analysis, electrophoresis and densitometry techniques, in the end the values obtained were analyzed, evaluated and compared statistically with data acquired in the questionnaire answered by participants. As a result, they have that there is significant difference (p=0,042) in average global methylation between the study groups, being the experimental with average of 51,44%, and the control with average of 50,17%. However no correlation was found between levels of methylation and the indoor radon dosed, being a correlation of p = 0,524, that was the main issue of the work, but still this result opens room for some h ypotheses and discussions, including on radioprotection. Three significant correlations were found, all inverses, these between methylation and 8-oxoguanosina dosed in the urine (p = 0,047 and Pearson = -0,291), physical exercise practice (p = 0,064 and Spearman = -0,269) and the kind of water consumed (p = 0,038 and Spearman = -0,329), this way it was possible to relate the epigenetics with oxidative damage and habits of life, respectively. However, these variables still explane for a few percent (R2= 0,189) of the variation in methylation levels, according to the multiple linear regression test. Finally, it was concluded that the initial question of the relationship between radon and global methylation has not been confirmed, but there is still much to be studied, thus leaving margins for future research and related new findings.Radônio; Lajes Pintadas/RN; Metilação Global; LINE-1; Câncer.Radon; Lajes Pintadas/RN; Global Methylation; LINE-1; Cancer.bachelorThesisGenética, Mutagênese, Toxicologia, Geologia